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Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential

BACKGROUND: Hypericum elegans is used in Bulgarian folk medicine for treatment of wounds, depression, gastrointestinal and bacterial diseases. OBJECTIVE: Recently, new natural benzophenones: Elegaphenone and O-glycosides: Hypericophenonoside, Neoannulatophenonoside and Elegaphenonoside as well as al...

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Autores principales: Zheleva-Dimitrova, Dimitrina, Nedialkov, Paraskev, Momekov, Georgi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798133/
https://www.ncbi.nlm.nih.gov/pubmed/24143038
http://dx.doi.org/10.4103/0973-1296.117846
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author Zheleva-Dimitrova, Dimitrina
Nedialkov, Paraskev
Momekov, Georgi
author_facet Zheleva-Dimitrova, Dimitrina
Nedialkov, Paraskev
Momekov, Georgi
author_sort Zheleva-Dimitrova, Dimitrina
collection PubMed
description BACKGROUND: Hypericum elegans is used in Bulgarian folk medicine for treatment of wounds, depression, gastrointestinal and bacterial diseases. OBJECTIVE: Recently, new natural benzophenones: Elegaphenone and O-glycosides: Hypericophenonoside, Neoannulatophenonoside and Elegaphenonoside as well as already known 7-Epiclusianone were isolated from the titled species. The aim of the present study was to evaluate the antioxidant and acetyl cholinesterase inhibitory potential of the isolated compounds. MATERIALS AND METHODS: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) free radicals, ferric reducing antioxidant power (FRAP) assay as well as inhibition of lipid peroxidation in linoleic acid system were used for determination of antioxidant activity. Modified Ellman's colorimetric method was carried out to assess the acetyl cholinesterase inhibition potential. Hyperoside and Galantamine hydrobromide were used as positive controls. RESULTS: Hypericophenonoside was found to possess the strongest DPPH radical scavenging activity (IC(50) = 181.85 ± 6.82 μM), while Neoannulatophenonoside showed the highest ABTS (IC(50) = 0.25 ± 0.005 μM) and lipid peroxidation inhibitor activity. FRAP activity was demonstrated only by prenylated aglycones – Elegaphenone [942.16 ± 4.03 μM Trolox Equivalent (TE)] and 7-Epiclusianone (642.95 ± 3.95 μM TE) and was stronger compared to the control Hyperoside (421.75 ± 9.29 μM TE). Elegaphenone and 7-Epiclusianone were found to possess moderate acetyl cholinesterase inhibitory potential with IC(50) values of 192.19 ± 3.54 μM and 142.97 ± 4.62 μM, respectively. CONCLUSION: The results obtained revealed that H. elegans is a potential natural source of bioactive compounds and benzophenones could be useful in therapy of free radical pathologies and neurodegenerative disorders.
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spelling pubmed-37981332013-10-18 Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential Zheleva-Dimitrova, Dimitrina Nedialkov, Paraskev Momekov, Georgi Pharmacogn Mag Original Article BACKGROUND: Hypericum elegans is used in Bulgarian folk medicine for treatment of wounds, depression, gastrointestinal and bacterial diseases. OBJECTIVE: Recently, new natural benzophenones: Elegaphenone and O-glycosides: Hypericophenonoside, Neoannulatophenonoside and Elegaphenonoside as well as already known 7-Epiclusianone were isolated from the titled species. The aim of the present study was to evaluate the antioxidant and acetyl cholinesterase inhibitory potential of the isolated compounds. MATERIALS AND METHODS: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) free radicals, ferric reducing antioxidant power (FRAP) assay as well as inhibition of lipid peroxidation in linoleic acid system were used for determination of antioxidant activity. Modified Ellman's colorimetric method was carried out to assess the acetyl cholinesterase inhibition potential. Hyperoside and Galantamine hydrobromide were used as positive controls. RESULTS: Hypericophenonoside was found to possess the strongest DPPH radical scavenging activity (IC(50) = 181.85 ± 6.82 μM), while Neoannulatophenonoside showed the highest ABTS (IC(50) = 0.25 ± 0.005 μM) and lipid peroxidation inhibitor activity. FRAP activity was demonstrated only by prenylated aglycones – Elegaphenone [942.16 ± 4.03 μM Trolox Equivalent (TE)] and 7-Epiclusianone (642.95 ± 3.95 μM TE) and was stronger compared to the control Hyperoside (421.75 ± 9.29 μM TE). Elegaphenone and 7-Epiclusianone were found to possess moderate acetyl cholinesterase inhibitory potential with IC(50) values of 192.19 ± 3.54 μM and 142.97 ± 4.62 μM, respectively. CONCLUSION: The results obtained revealed that H. elegans is a potential natural source of bioactive compounds and benzophenones could be useful in therapy of free radical pathologies and neurodegenerative disorders. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3798133/ /pubmed/24143038 http://dx.doi.org/10.4103/0973-1296.117846 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zheleva-Dimitrova, Dimitrina
Nedialkov, Paraskev
Momekov, Georgi
Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
title Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
title_full Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
title_fullStr Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
title_full_unstemmed Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
title_short Benzophenones from Hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
title_sort benzophenones from hypericum elegans with antioxidant and acetylcholinesterase inhibitory potential
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798133/
https://www.ncbi.nlm.nih.gov/pubmed/24143038
http://dx.doi.org/10.4103/0973-1296.117846
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