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Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome

Activity-dependent gene expression is central for sculpting neuronal connectivity in the brain. Despite the importance for synaptic plasticity, a comprehensive analysis of the temporal changes in the transcriptomic response to neuronal activity is lacking. In a genome wide survey we identified genes...

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Autores principales: Hermey, Guido, Mahlke, Claudia, Gutzmann, Jakob J., Schreiber, Jörg, Blüthgen, Nils, Kuhl, Dietmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798291/
https://www.ncbi.nlm.nih.gov/pubmed/24146943
http://dx.doi.org/10.1371/journal.pone.0076903
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author Hermey, Guido
Mahlke, Claudia
Gutzmann, Jakob J.
Schreiber, Jörg
Blüthgen, Nils
Kuhl, Dietmar
author_facet Hermey, Guido
Mahlke, Claudia
Gutzmann, Jakob J.
Schreiber, Jörg
Blüthgen, Nils
Kuhl, Dietmar
author_sort Hermey, Guido
collection PubMed
description Activity-dependent gene expression is central for sculpting neuronal connectivity in the brain. Despite the importance for synaptic plasticity, a comprehensive analysis of the temporal changes in the transcriptomic response to neuronal activity is lacking. In a genome wide survey we identified genes that were induced at 1, 4, 8, or 24 hours following neuronal activity in the hippocampus. According to their distinct expression kinetics we assigned these genes to five clusters, each containing approximately 200 genes. Using in situ hybridizations the regulated expression of 24 genes was validated. Apart from known activity-dependent genes our study reveals a large number of unknown induced genes with distinct expression kinetics. Among these we identified several genes with complex temporal expression patterns. Furthermore, our study provides examples for activity-induced exon switching in the coding region of genes and activity-induced alternative splicing of the 3′-UTR. One example is Zwint. In contrast to the constitutively expressed variant, the induced Zwint transcript harbors multiple regulatory elements in the 3′-UTR. Taken together, our study provides a comprehensive analysis of the transcriptomic response to neuronal activity and sheds new light on expression kinetics and alternative splicing events.
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spelling pubmed-37982912013-10-21 Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome Hermey, Guido Mahlke, Claudia Gutzmann, Jakob J. Schreiber, Jörg Blüthgen, Nils Kuhl, Dietmar PLoS One Research Article Activity-dependent gene expression is central for sculpting neuronal connectivity in the brain. Despite the importance for synaptic plasticity, a comprehensive analysis of the temporal changes in the transcriptomic response to neuronal activity is lacking. In a genome wide survey we identified genes that were induced at 1, 4, 8, or 24 hours following neuronal activity in the hippocampus. According to their distinct expression kinetics we assigned these genes to five clusters, each containing approximately 200 genes. Using in situ hybridizations the regulated expression of 24 genes was validated. Apart from known activity-dependent genes our study reveals a large number of unknown induced genes with distinct expression kinetics. Among these we identified several genes with complex temporal expression patterns. Furthermore, our study provides examples for activity-induced exon switching in the coding region of genes and activity-induced alternative splicing of the 3′-UTR. One example is Zwint. In contrast to the constitutively expressed variant, the induced Zwint transcript harbors multiple regulatory elements in the 3′-UTR. Taken together, our study provides a comprehensive analysis of the transcriptomic response to neuronal activity and sheds new light on expression kinetics and alternative splicing events. Public Library of Science 2013-10-17 /pmc/articles/PMC3798291/ /pubmed/24146943 http://dx.doi.org/10.1371/journal.pone.0076903 Text en © 2013 Hermey et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hermey, Guido
Mahlke, Claudia
Gutzmann, Jakob J.
Schreiber, Jörg
Blüthgen, Nils
Kuhl, Dietmar
Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome
title Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome
title_full Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome
title_fullStr Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome
title_full_unstemmed Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome
title_short Genome-Wide Profiling of the Activity-Dependent Hippocampal Transcriptome
title_sort genome-wide profiling of the activity-dependent hippocampal transcriptome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798291/
https://www.ncbi.nlm.nih.gov/pubmed/24146943
http://dx.doi.org/10.1371/journal.pone.0076903
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