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Efficacy of a Vaccine Formula against Tuberculosis in Cattle

“Test-and-slaughter” has been successful in industrialized countries to control and eradicate tuberculosis from cattle; however, this strategy is too expensive for developing nations, where the prevalence is especially high. Vaccination with the Calmette-Guérin (BCG) strain has been shown to protect...

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Autores principales: Canto Alarcon, Germinal J., Rubio Venegas, Yezenia, Bojorquez Narvaez, Luis, Pizano Martínez, Oscar E., García Casanova, Leticia, Sosa Gallegos, Susana, Nava Vargas, Alejandro, Olvera Ramírez, Andrea M., Milian Suazo, Feliciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799756/
https://www.ncbi.nlm.nih.gov/pubmed/24204624
http://dx.doi.org/10.1371/journal.pone.0076418
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author Canto Alarcon, Germinal J.
Rubio Venegas, Yezenia
Bojorquez Narvaez, Luis
Pizano Martínez, Oscar E.
García Casanova, Leticia
Sosa Gallegos, Susana
Nava Vargas, Alejandro
Olvera Ramírez, Andrea M.
Milian Suazo, Feliciano
author_facet Canto Alarcon, Germinal J.
Rubio Venegas, Yezenia
Bojorquez Narvaez, Luis
Pizano Martínez, Oscar E.
García Casanova, Leticia
Sosa Gallegos, Susana
Nava Vargas, Alejandro
Olvera Ramírez, Andrea M.
Milian Suazo, Feliciano
author_sort Canto Alarcon, Germinal J.
collection PubMed
description “Test-and-slaughter” has been successful in industrialized countries to control and eradicate tuberculosis from cattle; however, this strategy is too expensive for developing nations, where the prevalence is especially high. Vaccination with the Calmette-Guérin (BCG) strain has been shown to protect against the development of lesions in vaccinated animals: mouse, cattle and wildlife species. In this study, the immune response and the pathology of vaccinated (BCG-prime and BCG prime-CFP-boosted) and unvaccinated (controls) calves were evaluated under experimental settings. A 10(6) CFU dose of the BCG strain was inoculated subcutaneously on the neck to two groups of ten animas each. Thirty days after vaccination, one of the vaccinated groups was boosted with an M. bovis culture filtrate protein (CFP). Three months after vaccination, the three groups of animals were challenged with 5×10(5) CFU via intranasal by aerosol with a field strain of M. bovis. The immune response was monitored throughout the study. Protection was assessed based on immune response (IFN-g release) prechallenge, presence of visible lesions in lymph nodes and lungs at slaughter, and presence of bacilli in lymph nodes and lung samples in histological analysis. Vaccinated cattle, either with the BCG alone or with BCG and boosted with CFP showed higher IFN-g response, fewer lesions, and fewer bacilli per lesion than unvaccinated controls after challenge. Animals with low levels of IFN-g postvaccine-prechallenge showed more lesions than animals with high levels. Results from this study support the argument that vaccination could be incorporated into control programs to reduce the incidence of TB in cattle in countries with high prevalence.
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spelling pubmed-37997562013-11-07 Efficacy of a Vaccine Formula against Tuberculosis in Cattle Canto Alarcon, Germinal J. Rubio Venegas, Yezenia Bojorquez Narvaez, Luis Pizano Martínez, Oscar E. García Casanova, Leticia Sosa Gallegos, Susana Nava Vargas, Alejandro Olvera Ramírez, Andrea M. Milian Suazo, Feliciano PLoS One Research Article “Test-and-slaughter” has been successful in industrialized countries to control and eradicate tuberculosis from cattle; however, this strategy is too expensive for developing nations, where the prevalence is especially high. Vaccination with the Calmette-Guérin (BCG) strain has been shown to protect against the development of lesions in vaccinated animals: mouse, cattle and wildlife species. In this study, the immune response and the pathology of vaccinated (BCG-prime and BCG prime-CFP-boosted) and unvaccinated (controls) calves were evaluated under experimental settings. A 10(6) CFU dose of the BCG strain was inoculated subcutaneously on the neck to two groups of ten animas each. Thirty days after vaccination, one of the vaccinated groups was boosted with an M. bovis culture filtrate protein (CFP). Three months after vaccination, the three groups of animals were challenged with 5×10(5) CFU via intranasal by aerosol with a field strain of M. bovis. The immune response was monitored throughout the study. Protection was assessed based on immune response (IFN-g release) prechallenge, presence of visible lesions in lymph nodes and lungs at slaughter, and presence of bacilli in lymph nodes and lung samples in histological analysis. Vaccinated cattle, either with the BCG alone or with BCG and boosted with CFP showed higher IFN-g response, fewer lesions, and fewer bacilli per lesion than unvaccinated controls after challenge. Animals with low levels of IFN-g postvaccine-prechallenge showed more lesions than animals with high levels. Results from this study support the argument that vaccination could be incorporated into control programs to reduce the incidence of TB in cattle in countries with high prevalence. Public Library of Science 2013-10-18 /pmc/articles/PMC3799756/ /pubmed/24204624 http://dx.doi.org/10.1371/journal.pone.0076418 Text en © 2013 Canto Alarcon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Canto Alarcon, Germinal J.
Rubio Venegas, Yezenia
Bojorquez Narvaez, Luis
Pizano Martínez, Oscar E.
García Casanova, Leticia
Sosa Gallegos, Susana
Nava Vargas, Alejandro
Olvera Ramírez, Andrea M.
Milian Suazo, Feliciano
Efficacy of a Vaccine Formula against Tuberculosis in Cattle
title Efficacy of a Vaccine Formula against Tuberculosis in Cattle
title_full Efficacy of a Vaccine Formula against Tuberculosis in Cattle
title_fullStr Efficacy of a Vaccine Formula against Tuberculosis in Cattle
title_full_unstemmed Efficacy of a Vaccine Formula against Tuberculosis in Cattle
title_short Efficacy of a Vaccine Formula against Tuberculosis in Cattle
title_sort efficacy of a vaccine formula against tuberculosis in cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799756/
https://www.ncbi.nlm.nih.gov/pubmed/24204624
http://dx.doi.org/10.1371/journal.pone.0076418
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