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Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placen...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799759/ https://www.ncbi.nlm.nih.gov/pubmed/24205073 http://dx.doi.org/10.1371/journal.pone.0078025 |
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author | Wang, Fuqiang Shi, Zhonghua Wang, Ping You, Wei Liang, Gaolin |
author_facet | Wang, Fuqiang Shi, Zhonghua Wang, Ping You, Wei Liang, Gaolin |
author_sort | Wang, Fuqiang |
collection | PubMed |
description | To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia. |
format | Online Article Text |
id | pubmed-3799759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37997592013-11-07 Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies Wang, Fuqiang Shi, Zhonghua Wang, Ping You, Wei Liang, Gaolin PLoS One Research Article To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia. Public Library of Science 2013-10-18 /pmc/articles/PMC3799759/ /pubmed/24205073 http://dx.doi.org/10.1371/journal.pone.0078025 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Fuqiang Shi, Zhonghua Wang, Ping You, Wei Liang, Gaolin Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies |
title | Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies |
title_full | Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies |
title_fullStr | Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies |
title_full_unstemmed | Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies |
title_short | Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies |
title_sort | comparative proteome profile of human placenta from normal and preeclamptic pregnancies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799759/ https://www.ncbi.nlm.nih.gov/pubmed/24205073 http://dx.doi.org/10.1371/journal.pone.0078025 |
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