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Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placen...

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Detalles Bibliográficos
Autores principales: Wang, Fuqiang, Shi, Zhonghua, Wang, Ping, You, Wei, Liang, Gaolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799759/
https://www.ncbi.nlm.nih.gov/pubmed/24205073
http://dx.doi.org/10.1371/journal.pone.0078025
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author Wang, Fuqiang
Shi, Zhonghua
Wang, Ping
You, Wei
Liang, Gaolin
author_facet Wang, Fuqiang
Shi, Zhonghua
Wang, Ping
You, Wei
Liang, Gaolin
author_sort Wang, Fuqiang
collection PubMed
description To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia.
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spelling pubmed-37997592013-11-07 Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies Wang, Fuqiang Shi, Zhonghua Wang, Ping You, Wei Liang, Gaolin PLoS One Research Article To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia. Public Library of Science 2013-10-18 /pmc/articles/PMC3799759/ /pubmed/24205073 http://dx.doi.org/10.1371/journal.pone.0078025 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Fuqiang
Shi, Zhonghua
Wang, Ping
You, Wei
Liang, Gaolin
Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
title Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
title_full Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
title_fullStr Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
title_full_unstemmed Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
title_short Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies
title_sort comparative proteome profile of human placenta from normal and preeclamptic pregnancies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799759/
https://www.ncbi.nlm.nih.gov/pubmed/24205073
http://dx.doi.org/10.1371/journal.pone.0078025
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