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Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3
The mammalian hippocampus functions to encode and retrieve memories by transiently changing synaptic strengths, yet encoding in individual subregions for transmission between regions remains poorly understood. Toward the goal of better understanding the coding in the trisynaptic pathway from the den...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800815/ https://www.ncbi.nlm.nih.gov/pubmed/24155693 http://dx.doi.org/10.3389/fncir.2013.00165 |
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author | Brewer, Gregory J. Boehler, Michael D. Leondopulos, Stathis Pan, Liangbin Alagapan, Sankaraleengam DeMarse, Thomas B. Wheeler, Bruce C. |
author_facet | Brewer, Gregory J. Boehler, Michael D. Leondopulos, Stathis Pan, Liangbin Alagapan, Sankaraleengam DeMarse, Thomas B. Wheeler, Bruce C. |
author_sort | Brewer, Gregory J. |
collection | PubMed |
description | The mammalian hippocampus functions to encode and retrieve memories by transiently changing synaptic strengths, yet encoding in individual subregions for transmission between regions remains poorly understood. Toward the goal of better understanding the coding in the trisynaptic pathway from the dentate gyrus (DG) to the CA3 and CA1, we report a novel microfabricated device that divides a micro-electrode array into two compartments of separate hippocampal network subregions connected by axons that grow through 3 × 10 × 400 μm tunnels. Gene expression by qPCR demonstrated selective enrichment of separate DG, CA3, and CA1 subregions. Reconnection of DG to CA3 altered burst dynamics associated with marked enrichment of GAD67 in DG and GFAP in CA3. Surprisingly, DG axon spike propagation was preferentially unidirectional to the CA3 region at 0.5 m/s with little reverse transmission. Therefore, select hippocampal subregions intrinsically self-wire in anatomically appropriate patterns and maintain their distinct subregion phenotype without external inputs. |
format | Online Article Text |
id | pubmed-3800815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-38008152013-10-23 Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 Brewer, Gregory J. Boehler, Michael D. Leondopulos, Stathis Pan, Liangbin Alagapan, Sankaraleengam DeMarse, Thomas B. Wheeler, Bruce C. Front Neural Circuits Neuroscience The mammalian hippocampus functions to encode and retrieve memories by transiently changing synaptic strengths, yet encoding in individual subregions for transmission between regions remains poorly understood. Toward the goal of better understanding the coding in the trisynaptic pathway from the dentate gyrus (DG) to the CA3 and CA1, we report a novel microfabricated device that divides a micro-electrode array into two compartments of separate hippocampal network subregions connected by axons that grow through 3 × 10 × 400 μm tunnels. Gene expression by qPCR demonstrated selective enrichment of separate DG, CA3, and CA1 subregions. Reconnection of DG to CA3 altered burst dynamics associated with marked enrichment of GAD67 in DG and GFAP in CA3. Surprisingly, DG axon spike propagation was preferentially unidirectional to the CA3 region at 0.5 m/s with little reverse transmission. Therefore, select hippocampal subregions intrinsically self-wire in anatomically appropriate patterns and maintain their distinct subregion phenotype without external inputs. Frontiers Media S.A. 2013-10-21 /pmc/articles/PMC3800815/ /pubmed/24155693 http://dx.doi.org/10.3389/fncir.2013.00165 Text en Copyright © 2013 Brewer, Boehler, Leondopulos, Pan, Alagapan, DeMarse and Wheeler. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Brewer, Gregory J. Boehler, Michael D. Leondopulos, Stathis Pan, Liangbin Alagapan, Sankaraleengam DeMarse, Thomas B. Wheeler, Bruce C. Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 |
title | Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 |
title_full | Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 |
title_fullStr | Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 |
title_full_unstemmed | Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 |
title_short | Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3 |
title_sort | toward a self-wired active reconstruction of the hippocampal trisynaptic loop: dg-ca3 |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800815/ https://www.ncbi.nlm.nih.gov/pubmed/24155693 http://dx.doi.org/10.3389/fncir.2013.00165 |
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