OA01.11. Toxicity study and testicular regeneration property of swarna vanga

PURPOSE: Swarna-Vanga (SV) is a famous Kupipakva metallic preparation, which are therapeutically used as rejuvenator, anti-diabetic, and for testicular regeneration (Spermatogenesis) etc since 18th century. It contains Parad (mercury-Hg), Vanga (tin-Sn) and Gandhaka (Sulphur-S) in major amounts and our ancient author use different proportion of mercury in SV with respect to tin. So, in present study an attempt has been made to evaluate testicular regeneration property of SV with its safety and efficacy on albino rats. METHOD: 1. Pharmaceutical Study: Preparation of SV in different proportion of Sn:Hg as 1:1,1:1/2, 1:1/4, 1:1/6. 2. Experimental Study: a) Toxicity study of SV for short (14 days) and long(40 days) duration in different doses, b) Regeneration property of SV on induced CdCl2 (Cadmium Chloride) testicular germinal epithelium of albinorats. RESULT: SV is a metallic preparation which contains mercury, tin and sulphur in major amount and the best product of SV is made by half of the mercury with the tin. SV does not show any toxic effects on therapeutic dose (12.5-25 gm/body weight of albino rats) but show some toxic effects on higher dose in longer duration. The ability of SV in generation of testicular germinal epithelium is seen on partial damaged testis which is produced by inducing CdCl2. CONCLUSION: 1) The presence of mercury at least in half proportion to tin in is necessary in making the SV of good standards, 2) SV is not toxic in therapeutic dose and is slightly toxic on higher doses in long duration, 3) SV has the property to regenerate partially damaged testicular tissue.