PA01.20. Acute and sub-acute toxicological study of rasa manikya prepared with classical-modified and adopted method

PURPOSE: Rasamanikya a very famous preparation with different pharmaceutical methods used in various diseases since long, but in todays modern era it requires toxicological updating to understand the drug by carrying out animal experimentation so as to provide scientific data and statistical validation for its safe and efficacious action. The main intention of conducting this study was to find out and understand various toxic effects produced by the drug Rasamanikya in the present era when it is prepared with three different procedures though with the same drug Haratala (Orpiment). METHOD: Toxicological study of Rasamanikya prepared by three methods was subjected on Wistar stain albino rats for acute toxicity study by taking up and down/staircase method and sub acute toxicity study by giving ten times that of the therapeutic dose. After 21 days duration of drug schedule albino rats from each group were anesthetized and sacrificed. Liver function test was assessed and the tissues of Kidney, Liver and Brain were subjected for Histopathological study. RESULT: The LFT report revealed in all the three groups there was mild increase in the SGOT, ALP and Tot. Bilirubin values respectively with statistical significant result indicative of mild liver dysfunction and bile duct injury which may be correlated with the mild fatty changes of liver cells. Histopathological reports showed minor toxic effects but with no any remarkable damage to Brain, Liver & Kidney and overall findings were suggestive of normal appearance. CONCLUSION: Acute and Sub Acute study showed no immediate and evident toxic signs and mortality. Rasamanikya prepared with adopted method showed mild toxicity but with an attempt of healing and repair. Histopathological and LFT study showed mild to moderate fatty changes which were statistically significant. Overall these toxic effects of mild to moderate changes in rat cells with correspondence to human cells may be considered as non specific and reversible.