Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, bei...

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Detalles Bibliográficos
Autores principales: Moraes, Paula Mendonça, Perin, Luana Martins, Júnior, Abelardo Silva, Nero, Luís Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804185/
https://www.ncbi.nlm.nih.gov/pubmed/24159291
http://dx.doi.org/10.1590/S1517-83822013000100015
Descripción
Sumario:Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.

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