Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, bei...

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Detalles Bibliográficos
Autores principales: Moraes, Paula Mendonça, Perin, Luana Martins, Júnior, Abelardo Silva, Nero, Luís Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804185/
https://www.ncbi.nlm.nih.gov/pubmed/24159291
http://dx.doi.org/10.1590/S1517-83822013000100015
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author Moraes, Paula Mendonça
Perin, Luana Martins
Júnior, Abelardo Silva
Nero, Luís Augusto
author_facet Moraes, Paula Mendonça
Perin, Luana Martins
Júnior, Abelardo Silva
Nero, Luís Augusto
author_sort Moraes, Paula Mendonça
collection PubMed
description Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
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spelling pubmed-38041852013-10-24 Comparison of phenotypic and molecular tests to identify lactic acid bacteria Moraes, Paula Mendonça Perin, Luana Martins Júnior, Abelardo Silva Nero, Luís Augusto Braz J Microbiol Short Communication Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable. Brazilian Society of Microbiology 2013-05-31 /pmc/articles/PMC3804185/ /pubmed/24159291 http://dx.doi.org/10.1590/S1517-83822013000100015 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Short Communication
Moraes, Paula Mendonça
Perin, Luana Martins
Júnior, Abelardo Silva
Nero, Luís Augusto
Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_full Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_fullStr Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_full_unstemmed Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_short Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_sort comparison of phenotypic and molecular tests to identify lactic acid bacteria
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804185/
https://www.ncbi.nlm.nih.gov/pubmed/24159291
http://dx.doi.org/10.1590/S1517-83822013000100015
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