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Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules

To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (co...

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Autores principales: da Silva, Karla Patrícia Chaves, de Campos Takaki, Galba Maria, da Silva, Leonildo Bento Galiza, Saukas, Tomoe Noda, Santos, André Souza, Mota, Rinaldo Aparecido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804197/
https://www.ncbi.nlm.nih.gov/pubmed/24159303
http://dx.doi.org/10.1590/S1517-83822013005000022
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author da Silva, Karla Patrícia Chaves
de Campos Takaki, Galba Maria
da Silva, Leonildo Bento Galiza
Saukas, Tomoe Noda
Santos, André Souza
Mota, Rinaldo Aparecido
author_facet da Silva, Karla Patrícia Chaves
de Campos Takaki, Galba Maria
da Silva, Leonildo Bento Galiza
Saukas, Tomoe Noda
Santos, André Souza
Mota, Rinaldo Aparecido
author_sort da Silva, Karla Patrícia Chaves
collection PubMed
description To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichloroacetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no inflammatory reaction at the site of inoculation of PPD-mallein. This immunogen produced in Brazil and still being tested was effective for identifying the infection in true positive animals and excluding the truly negative ones, being a new possibility for diagnosis and control of glanders
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spelling pubmed-38041972013-10-24 Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules da Silva, Karla Patrícia Chaves de Campos Takaki, Galba Maria da Silva, Leonildo Bento Galiza Saukas, Tomoe Noda Santos, André Souza Mota, Rinaldo Aparecido Braz J Microbiol Short Communication To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichloroacetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no inflammatory reaction at the site of inoculation of PPD-mallein. This immunogen produced in Brazil and still being tested was effective for identifying the infection in true positive animals and excluding the truly negative ones, being a new possibility for diagnosis and control of glanders Brazilian Society of Microbiology 2013-05-07 /pmc/articles/PMC3804197/ /pubmed/24159303 http://dx.doi.org/10.1590/S1517-83822013005000022 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Short Communication
da Silva, Karla Patrícia Chaves
de Campos Takaki, Galba Maria
da Silva, Leonildo Bento Galiza
Saukas, Tomoe Noda
Santos, André Souza
Mota, Rinaldo Aparecido
Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
title Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
title_full Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
title_fullStr Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
title_full_unstemmed Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
title_short Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
title_sort assessment of the effectiveness of the ppd-mallein produced in brazil for diagnosing glanders in mules
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804197/
https://www.ncbi.nlm.nih.gov/pubmed/24159303
http://dx.doi.org/10.1590/S1517-83822013005000022
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