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EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection
Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Microbiology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804208/ https://www.ncbi.nlm.nih.gov/pubmed/24159314 http://dx.doi.org/10.1590/S1517-83822013005000019 |
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author | Zhou, Huiqin Du, Hong Zhang, Haifang Shen, Haiying Yan, Ruhong He, Yun Wang, Min Zhu, Xueming |
author_facet | Zhou, Huiqin Du, Hong Zhang, Haifang Shen, Haiying Yan, Ruhong He, Yun Wang, Min Zhu, Xueming |
author_sort | Zhou, Huiqin |
collection | PubMed |
description | Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine against S. aureus has become one of the world’s hot spots once again. The key issue of developing the vaccine of S. aureus is how to find an ideal key pathogenic gene of S. aureus. It was previously suggested that EsxA might be a very important factor in S. aureus abscess formation in mice, but clinical experimental evidence was lacking. We therefore expressed EsxA protein through prokaryotic expression system and purified EsxA protein by Ni-affinity chromatography. ELISA was used to detect the anti-EsxA antibodies in sera of 78 patients with S. aureus infection and results showed that the anti-EsxA antibodies were positive in the sera of 19 patients. We further analyzed the EsxA positive antibodies related strains by antimicrobial susceptibility assay and found that all of the corresponding strains were multi-drug resistant. Among those multi-drug resistant strains, 73.7% were resistant to MRSA. The results indicated EsxA is very important in the pathogenesis of S. aureus. We suggested that the EsxA is very valuable as vaccine candidate target antigens for prevention and control of S. aureus infection. |
format | Online Article Text |
id | pubmed-3804208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Brazilian Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-38042082013-10-24 EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection Zhou, Huiqin Du, Hong Zhang, Haifang Shen, Haiying Yan, Ruhong He, Yun Wang, Min Zhu, Xueming Braz J Microbiol Research Paper Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine against S. aureus has become one of the world’s hot spots once again. The key issue of developing the vaccine of S. aureus is how to find an ideal key pathogenic gene of S. aureus. It was previously suggested that EsxA might be a very important factor in S. aureus abscess formation in mice, but clinical experimental evidence was lacking. We therefore expressed EsxA protein through prokaryotic expression system and purified EsxA protein by Ni-affinity chromatography. ELISA was used to detect the anti-EsxA antibodies in sera of 78 patients with S. aureus infection and results showed that the anti-EsxA antibodies were positive in the sera of 19 patients. We further analyzed the EsxA positive antibodies related strains by antimicrobial susceptibility assay and found that all of the corresponding strains were multi-drug resistant. Among those multi-drug resistant strains, 73.7% were resistant to MRSA. The results indicated EsxA is very important in the pathogenesis of S. aureus. We suggested that the EsxA is very valuable as vaccine candidate target antigens for prevention and control of S. aureus infection. Brazilian Society of Microbiology 2013-04-09 /pmc/articles/PMC3804208/ /pubmed/24159314 http://dx.doi.org/10.1590/S1517-83822013005000019 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Zhou, Huiqin Du, Hong Zhang, Haifang Shen, Haiying Yan, Ruhong He, Yun Wang, Min Zhu, Xueming EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection |
title | EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection |
title_full | EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection |
title_fullStr | EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection |
title_full_unstemmed | EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection |
title_short | EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection |
title_sort | esxa might as a virulence factor induce antibodies in patients with staphylococcus aureus infection |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804208/ https://www.ncbi.nlm.nih.gov/pubmed/24159314 http://dx.doi.org/10.1590/S1517-83822013005000019 |
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