Cargando…

Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units

The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD(600)) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD(600) measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We...

Descripción completa

Detalles Bibliográficos
Autores principales: Peñuelas-Urquides, Katia, Villarreal-Treviño, Licet, Silva-Ramírez, Beatriz, Rivadeneyra-Espinoza, Liliana, Said-Fernández, Salvador, de León, Mario Bermúdez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804212/
https://www.ncbi.nlm.nih.gov/pubmed/24159318
http://dx.doi.org/10.1590/S1517-83822013000100042
_version_ 1782288122092978176
author Peñuelas-Urquides, Katia
Villarreal-Treviño, Licet
Silva-Ramírez, Beatriz
Rivadeneyra-Espinoza, Liliana
Said-Fernández, Salvador
de León, Mario Bermúdez
author_facet Peñuelas-Urquides, Katia
Villarreal-Treviño, Licet
Silva-Ramírez, Beatriz
Rivadeneyra-Espinoza, Liliana
Said-Fernández, Salvador
de León, Mario Bermúdez
author_sort Peñuelas-Urquides, Katia
collection PubMed
description The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD(600)) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD(600) measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD(600) units.
format Online
Article
Text
id pubmed-3804212
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Brazilian Society of Microbiology
record_format MEDLINE/PubMed
spelling pubmed-38042122013-10-24 Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units Peñuelas-Urquides, Katia Villarreal-Treviño, Licet Silva-Ramírez, Beatriz Rivadeneyra-Espinoza, Liliana Said-Fernández, Salvador de León, Mario Bermúdez Braz J Microbiol Short Communication The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD(600)) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD(600) measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD(600) units. Brazilian Society of Microbiology 2013-05-31 /pmc/articles/PMC3804212/ /pubmed/24159318 http://dx.doi.org/10.1590/S1517-83822013000100042 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Short Communication
Peñuelas-Urquides, Katia
Villarreal-Treviño, Licet
Silva-Ramírez, Beatriz
Rivadeneyra-Espinoza, Liliana
Said-Fernández, Salvador
de León, Mario Bermúdez
Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units
title Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units
title_full Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units
title_fullStr Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units
title_full_unstemmed Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units
title_short Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units
title_sort measuring of mycobacterium tuberculosis growth. a correlation of the optical measurements with colony forming units
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804212/
https://www.ncbi.nlm.nih.gov/pubmed/24159318
http://dx.doi.org/10.1590/S1517-83822013000100042
work_keys_str_mv AT penuelasurquideskatia measuringofmycobacteriumtuberculosisgrowthacorrelationoftheopticalmeasurementswithcolonyformingunits
AT villarrealtrevinolicet measuringofmycobacteriumtuberculosisgrowthacorrelationoftheopticalmeasurementswithcolonyformingunits
AT silvaramirezbeatriz measuringofmycobacteriumtuberculosisgrowthacorrelationoftheopticalmeasurementswithcolonyformingunits
AT rivadeneyraespinozaliliana measuringofmycobacteriumtuberculosisgrowthacorrelationoftheopticalmeasurementswithcolonyformingunits
AT saidfernandezsalvador measuringofmycobacteriumtuberculosisgrowthacorrelationoftheopticalmeasurementswithcolonyformingunits
AT deleonmariobermudez measuringofmycobacteriumtuberculosisgrowthacorrelationoftheopticalmeasurementswithcolonyformingunits