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The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India

Objective. Sickle cell disease has variable clinical manifestations. Activation of neutrophils plays an important role in the initiation and propagation of vaso occlusive crises which can be analysed by determining the expression of neutrophil antigens such as CD16, CD32, and CD62L. The common FcγR...

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Autores principales: Kangne, Harshada K., Jijina, Farah F., Italia, Yazdi M., Jain, Dipti L., Nadkarni, Anita H., Gupta, Maya, Pradhan, Vandana, Mukesh, Rati D., Ghosh, Kanjaksha K., Colah, Roshan B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804363/
https://www.ncbi.nlm.nih.gov/pubmed/24191245
http://dx.doi.org/10.1155/2013/457656
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author Kangne, Harshada K.
Jijina, Farah F.
Italia, Yazdi M.
Jain, Dipti L.
Nadkarni, Anita H.
Gupta, Maya
Pradhan, Vandana
Mukesh, Rati D.
Ghosh, Kanjaksha K.
Colah, Roshan B.
author_facet Kangne, Harshada K.
Jijina, Farah F.
Italia, Yazdi M.
Jain, Dipti L.
Nadkarni, Anita H.
Gupta, Maya
Pradhan, Vandana
Mukesh, Rati D.
Ghosh, Kanjaksha K.
Colah, Roshan B.
author_sort Kangne, Harshada K.
collection PubMed
description Objective. Sickle cell disease has variable clinical manifestations. Activation of neutrophils plays an important role in the initiation and propagation of vaso occlusive crises which can be analysed by determining the expression of neutrophil antigens such as CD16, CD32, and CD62L. The common FcγR polymorphisms (FcγRIIA and FcγRIIIB) are considered to influence clinical presentation. This study focuses on distribution of FcγR polymorphisms and their association with neutrophil activity among the patients from western India. Methods. In this paper 127 sickle cell anemia patients and 58 patients with sickle-β-thalassemia (median age 12 ± 8.58 years) with variable clinical phenotypes along with 175 normals were investigated. FcγRs polymorphisms were analysed by RFLP and AS-PCR. Activation of neutrophils was measured by flow cytometry. Results. The genotypic frequency of the H/R genotype of FcγRIIA and the NA1/NA1 genotype of FcγRIIIB was significantly decreased in patients compared to normals (P-0.0074, P-0.0471, resp.). We found a significant difference in the expression of CD32 and CD62L among the patients as against normals. A significantly higher expression of CD32 was seen in the milder patients with the H/H genotype (P-0.0231), whereas the expression of CD16 was higher in severe patients with the NA2/NA2 genotype (P-0.0312). Conclusion. The two FcγR polymorphisms had significant association with variable phenotypes of sickle cell disease. The expression of CD62L decreased in our patients indicating activation of neutrophils.
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spelling pubmed-38043632013-11-04 The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India Kangne, Harshada K. Jijina, Farah F. Italia, Yazdi M. Jain, Dipti L. Nadkarni, Anita H. Gupta, Maya Pradhan, Vandana Mukesh, Rati D. Ghosh, Kanjaksha K. Colah, Roshan B. Biomed Res Int Research Article Objective. Sickle cell disease has variable clinical manifestations. Activation of neutrophils plays an important role in the initiation and propagation of vaso occlusive crises which can be analysed by determining the expression of neutrophil antigens such as CD16, CD32, and CD62L. The common FcγR polymorphisms (FcγRIIA and FcγRIIIB) are considered to influence clinical presentation. This study focuses on distribution of FcγR polymorphisms and their association with neutrophil activity among the patients from western India. Methods. In this paper 127 sickle cell anemia patients and 58 patients with sickle-β-thalassemia (median age 12 ± 8.58 years) with variable clinical phenotypes along with 175 normals were investigated. FcγRs polymorphisms were analysed by RFLP and AS-PCR. Activation of neutrophils was measured by flow cytometry. Results. The genotypic frequency of the H/R genotype of FcγRIIA and the NA1/NA1 genotype of FcγRIIIB was significantly decreased in patients compared to normals (P-0.0074, P-0.0471, resp.). We found a significant difference in the expression of CD32 and CD62L among the patients as against normals. A significantly higher expression of CD32 was seen in the milder patients with the H/H genotype (P-0.0231), whereas the expression of CD16 was higher in severe patients with the NA2/NA2 genotype (P-0.0312). Conclusion. The two FcγR polymorphisms had significant association with variable phenotypes of sickle cell disease. The expression of CD62L decreased in our patients indicating activation of neutrophils. Hindawi Publishing Corporation 2013 2013-09-29 /pmc/articles/PMC3804363/ /pubmed/24191245 http://dx.doi.org/10.1155/2013/457656 Text en Copyright © 2013 Harshada K. Kangne et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kangne, Harshada K.
Jijina, Farah F.
Italia, Yazdi M.
Jain, Dipti L.
Nadkarni, Anita H.
Gupta, Maya
Pradhan, Vandana
Mukesh, Rati D.
Ghosh, Kanjaksha K.
Colah, Roshan B.
The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
title The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
title_full The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
title_fullStr The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
title_full_unstemmed The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
title_short The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
title_sort fc receptor polymorphisms and expression of neutrophil activation markers in patients with sickle cell disease from western india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804363/
https://www.ncbi.nlm.nih.gov/pubmed/24191245
http://dx.doi.org/10.1155/2013/457656
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