Cargando…

Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?

Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between...

Descripción completa

Detalles Bibliográficos
Autores principales: Parkitny, Luke, McAuley, James H., Kelly, Patrick J., Di Pietro, Flavia, Cameron, Barbara, Moseley, G. Lorimer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804442/
https://www.ncbi.nlm.nih.gov/pubmed/24191133
http://dx.doi.org/10.1155/2013/890706
_version_ 1782288144632119296
author Parkitny, Luke
McAuley, James H.
Kelly, Patrick J.
Di Pietro, Flavia
Cameron, Barbara
Moseley, G. Lorimer
author_facet Parkitny, Luke
McAuley, James H.
Kelly, Patrick J.
Di Pietro, Flavia
Cameron, Barbara
Moseley, G. Lorimer
author_sort Parkitny, Luke
collection PubMed
description Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between concentrations of cytokines in plasma and serum, except for MIP1β. A thaw condition significantly changed the concentrations of most cytokines, but serum appeared less affected by this than plasma was. Closer examination using Bland-Altman analyses revealed that for each comparison, agreement was moderately good for many cytokine concentrations. This is important because measures of agreement must be interpreted based on the required precision, which may differ between clinical and research demands. We also identified that for some cytokines, the relationship between serum and plasma is affected by concentration, thus advocating for the use of appropriate methods when performing such comparisons in studies such as systematic reviews and meta-analyses.
format Online
Article
Text
id pubmed-3804442
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-38044422013-11-04 Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? Parkitny, Luke McAuley, James H. Kelly, Patrick J. Di Pietro, Flavia Cameron, Barbara Moseley, G. Lorimer Mediators Inflamm Research Article Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between concentrations of cytokines in plasma and serum, except for MIP1β. A thaw condition significantly changed the concentrations of most cytokines, but serum appeared less affected by this than plasma was. Closer examination using Bland-Altman analyses revealed that for each comparison, agreement was moderately good for many cytokine concentrations. This is important because measures of agreement must be interpreted based on the required precision, which may differ between clinical and research demands. We also identified that for some cytokines, the relationship between serum and plasma is affected by concentration, thus advocating for the use of appropriate methods when performing such comparisons in studies such as systematic reviews and meta-analyses. Hindawi Publishing Corporation 2013 2013-09-28 /pmc/articles/PMC3804442/ /pubmed/24191133 http://dx.doi.org/10.1155/2013/890706 Text en Copyright © 2013 Luke Parkitny et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Parkitny, Luke
McAuley, James H.
Kelly, Patrick J.
Di Pietro, Flavia
Cameron, Barbara
Moseley, G. Lorimer
Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
title Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
title_full Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
title_fullStr Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
title_full_unstemmed Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
title_short Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
title_sort multiplex cytokine concentration measurement: how much do the medium and handling matter?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804442/
https://www.ncbi.nlm.nih.gov/pubmed/24191133
http://dx.doi.org/10.1155/2013/890706
work_keys_str_mv AT parkitnyluke multiplexcytokineconcentrationmeasurementhowmuchdothemediumandhandlingmatter
AT mcauleyjamesh multiplexcytokineconcentrationmeasurementhowmuchdothemediumandhandlingmatter
AT kellypatrickj multiplexcytokineconcentrationmeasurementhowmuchdothemediumandhandlingmatter
AT dipietroflavia multiplexcytokineconcentrationmeasurementhowmuchdothemediumandhandlingmatter
AT cameronbarbara multiplexcytokineconcentrationmeasurementhowmuchdothemediumandhandlingmatter
AT moseleyglorimer multiplexcytokineconcentrationmeasurementhowmuchdothemediumandhandlingmatter