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Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization

As well as killing pest insects, the rhizosphere competent insect-pathogenic fungus Metarhizium robertsii also boosts plant growth by providing nitrogenous nutrients and increasing resistance to plant pathogens. Plant roots secrete abundant nutrients but little is known about their utilization by Me...

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Autores principales: Liao, Xinggang, Fang, Weiguo, Lin, Liangcai, Lu, Hsiao-Ling, Leger, Raymond J. St.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804458/
https://www.ncbi.nlm.nih.gov/pubmed/24205119
http://dx.doi.org/10.1371/journal.pone.0078118
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author Liao, Xinggang
Fang, Weiguo
Lin, Liangcai
Lu, Hsiao-Ling
Leger, Raymond J. St.
author_facet Liao, Xinggang
Fang, Weiguo
Lin, Liangcai
Lu, Hsiao-Ling
Leger, Raymond J. St.
author_sort Liao, Xinggang
collection PubMed
description As well as killing pest insects, the rhizosphere competent insect-pathogenic fungus Metarhizium robertsii also boosts plant growth by providing nitrogenous nutrients and increasing resistance to plant pathogens. Plant roots secrete abundant nutrients but little is known about their utilization by Metarhizium spp. and the mechanistic basis of Metarhizium-plant associations. We report here that M. robertsii produces an extracellular invertase (MrInv) on plant roots. Deletion of MrInv (⊿MrInv) reduced M. robertsii growth on sucrose and rhizospheric exudates but increased colonization of Panicum virgatum and Arabidopsis thaliana roots. This could be accounted for by a reduction in carbon catabolite repression in ⊿MrInv increasing production of plant cell wall-degrading depolymerases. A non-rhizosphere competent scarab beetle specialist Metarhizium majus lacks invertase which suggests that rhizospheric competence may be related to the sugar metabolism of different Metarhizium species.
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spelling pubmed-38044582013-11-07 Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization Liao, Xinggang Fang, Weiguo Lin, Liangcai Lu, Hsiao-Ling Leger, Raymond J. St. PLoS One Research Article As well as killing pest insects, the rhizosphere competent insect-pathogenic fungus Metarhizium robertsii also boosts plant growth by providing nitrogenous nutrients and increasing resistance to plant pathogens. Plant roots secrete abundant nutrients but little is known about their utilization by Metarhizium spp. and the mechanistic basis of Metarhizium-plant associations. We report here that M. robertsii produces an extracellular invertase (MrInv) on plant roots. Deletion of MrInv (⊿MrInv) reduced M. robertsii growth on sucrose and rhizospheric exudates but increased colonization of Panicum virgatum and Arabidopsis thaliana roots. This could be accounted for by a reduction in carbon catabolite repression in ⊿MrInv increasing production of plant cell wall-degrading depolymerases. A non-rhizosphere competent scarab beetle specialist Metarhizium majus lacks invertase which suggests that rhizospheric competence may be related to the sugar metabolism of different Metarhizium species. Public Library of Science 2013-10-21 /pmc/articles/PMC3804458/ /pubmed/24205119 http://dx.doi.org/10.1371/journal.pone.0078118 Text en © 2013 Liao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liao, Xinggang
Fang, Weiguo
Lin, Liangcai
Lu, Hsiao-Ling
Leger, Raymond J. St.
Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization
title Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization
title_full Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization
title_fullStr Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization
title_full_unstemmed Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization
title_short Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization
title_sort metarhizium robertsii produces an extracellular invertase (mrinv) that plays a pivotal role in rhizospheric interactions and root colonization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804458/
https://www.ncbi.nlm.nih.gov/pubmed/24205119
http://dx.doi.org/10.1371/journal.pone.0078118
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