Cargando…

The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus

It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus...

Descripción completa

Detalles Bibliográficos
Autores principales: Piedrafita, David, Preston, Sarah, Kemp, Joanna, de Veer, Michael, Sherrard, Jayne, Kraska, Troy, Elhay, Martin, Meeusen, Els
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804492/
https://www.ncbi.nlm.nih.gov/pubmed/24205209
http://dx.doi.org/10.1371/journal.pone.0078357
_version_ 1782288155400994816
author Piedrafita, David
Preston, Sarah
Kemp, Joanna
de Veer, Michael
Sherrard, Jayne
Kraska, Troy
Elhay, Martin
Meeusen, Els
author_facet Piedrafita, David
Preston, Sarah
Kemp, Joanna
de Veer, Michael
Sherrard, Jayne
Kraska, Troy
Elhay, Martin
Meeusen, Els
author_sort Piedrafita, David
collection PubMed
description It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus antigen, HcsL3. Using a suboptimal antigen dose, aluminium adjuvant was shown to reduce the cumulative faecal egg counts (cFEC) and worm burden by 23% and 25% respectively, in agreement with a previous study. The addition of Quil A to the aluminium-adjuvanted vaccine brought cFEC back to control levels. Vaccination with the adjuvant DEAE-dextran almost doubled the protection compared to the aluminium-adjuvanted vaccine resulting in 40% and 41% reduction in cFEC and worm counts compared to controls. Examination of skin responses following i.d. injection of exsheathed L3, revealed that cFEC was negatively correlated with wheal size and tissue eosinophils for the DEAE-dextran and aluminium-adjuvanted groups respectively. These studies have for the first time shown the potential of DEAE-dextran adjuvant for helminth vaccines, and discovered significant cellular correlates of vaccine-induced protection.
format Online
Article
Text
id pubmed-3804492
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38044922013-11-07 The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus Piedrafita, David Preston, Sarah Kemp, Joanna de Veer, Michael Sherrard, Jayne Kraska, Troy Elhay, Martin Meeusen, Els PLoS One Research Article It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus antigen, HcsL3. Using a suboptimal antigen dose, aluminium adjuvant was shown to reduce the cumulative faecal egg counts (cFEC) and worm burden by 23% and 25% respectively, in agreement with a previous study. The addition of Quil A to the aluminium-adjuvanted vaccine brought cFEC back to control levels. Vaccination with the adjuvant DEAE-dextran almost doubled the protection compared to the aluminium-adjuvanted vaccine resulting in 40% and 41% reduction in cFEC and worm counts compared to controls. Examination of skin responses following i.d. injection of exsheathed L3, revealed that cFEC was negatively correlated with wheal size and tissue eosinophils for the DEAE-dextran and aluminium-adjuvanted groups respectively. These studies have for the first time shown the potential of DEAE-dextran adjuvant for helminth vaccines, and discovered significant cellular correlates of vaccine-induced protection. Public Library of Science 2013-10-21 /pmc/articles/PMC3804492/ /pubmed/24205209 http://dx.doi.org/10.1371/journal.pone.0078357 Text en © 2013 Piedrafita et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Piedrafita, David
Preston, Sarah
Kemp, Joanna
de Veer, Michael
Sherrard, Jayne
Kraska, Troy
Elhay, Martin
Meeusen, Els
The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus
title The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus
title_full The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus
title_fullStr The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus
title_full_unstemmed The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus
title_short The Effect of Different Adjuvants on Immune Parameters and Protection following Vaccination of Sheep with a Larval-Specific Antigen of the Gastrointestinal Nematode, Haemonchus contortus
title_sort effect of different adjuvants on immune parameters and protection following vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, haemonchus contortus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804492/
https://www.ncbi.nlm.nih.gov/pubmed/24205209
http://dx.doi.org/10.1371/journal.pone.0078357
work_keys_str_mv AT piedrafitadavid theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT prestonsarah theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT kempjoanna theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT deveermichael theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT sherrardjayne theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT kraskatroy theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT elhaymartin theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT meeusenels theeffectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT piedrafitadavid effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT prestonsarah effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT kempjoanna effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT deveermichael effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT sherrardjayne effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT kraskatroy effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT elhaymartin effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus
AT meeusenels effectofdifferentadjuvantsonimmuneparametersandprotectionfollowingvaccinationofsheepwithalarvalspecificantigenofthegastrointestinalnematodehaemonchuscontortus