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Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells
Oral squamous cell carcinoma (OSCC) has a tendency to migrate and metastasize. WNT1-inducible signaling pathway protein 1 (WISP-1) is a cysteine-rich protein that belongs to the Cyr61, CTGF, Nov (CCN) family of matrix cellular proteins. The effect of WISP-1 on human OSCC cells, however, is unknown....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804520/ https://www.ncbi.nlm.nih.gov/pubmed/24205072 http://dx.doi.org/10.1371/journal.pone.0078022 |
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author | Chuang, Jing-Yuan Chang, An-Chen Chiang, I-Ping Tsai, Ming-Hsui Tang, Chih-Hsin |
author_facet | Chuang, Jing-Yuan Chang, An-Chen Chiang, I-Ping Tsai, Ming-Hsui Tang, Chih-Hsin |
author_sort | Chuang, Jing-Yuan |
collection | PubMed |
description | Oral squamous cell carcinoma (OSCC) has a tendency to migrate and metastasize. WNT1-inducible signaling pathway protein 1 (WISP-1) is a cysteine-rich protein that belongs to the Cyr61, CTGF, Nov (CCN) family of matrix cellular proteins. The effect of WISP-1 on human OSCC cells, however, is unknown. Here, we showed that WISP-1 increased cell migration and intercellular adhesion molecule-1 (ICAM-1) expression in OSCC cells. Pretreatment of cells with integrin αvβ3 monoclonal antibody (mAb) significantly abolished WISP-1–induced cell migration and ICAM-1 expression. On the other hand, WISP-1–mediated cell motility and ICAM-1 upregulation were attenuated by ASK1, JNK, and p38 inhibitor. Furthermore, WISP-1 also enhanced activator protein 1 (AP-1) activation, and the integrin αvβ3 mAb, and ASK1, JNK, and p38 inhibitors reduced WISP-1–mediated AP-1 activation. Moreover, WISP-1 and ICAM-1 expression correlated with the tumor stage of patients with OSCC. Our results indicate that WISP-1 enhances the migration of OSCC cells by increasing ICAM-1 expression through the αvβ3 integrin receptor and the ASK1, JNK/p38, and AP-1 signal transduction pathways. |
format | Online Article Text |
id | pubmed-3804520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38045202013-11-07 Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells Chuang, Jing-Yuan Chang, An-Chen Chiang, I-Ping Tsai, Ming-Hsui Tang, Chih-Hsin PLoS One Research Article Oral squamous cell carcinoma (OSCC) has a tendency to migrate and metastasize. WNT1-inducible signaling pathway protein 1 (WISP-1) is a cysteine-rich protein that belongs to the Cyr61, CTGF, Nov (CCN) family of matrix cellular proteins. The effect of WISP-1 on human OSCC cells, however, is unknown. Here, we showed that WISP-1 increased cell migration and intercellular adhesion molecule-1 (ICAM-1) expression in OSCC cells. Pretreatment of cells with integrin αvβ3 monoclonal antibody (mAb) significantly abolished WISP-1–induced cell migration and ICAM-1 expression. On the other hand, WISP-1–mediated cell motility and ICAM-1 upregulation were attenuated by ASK1, JNK, and p38 inhibitor. Furthermore, WISP-1 also enhanced activator protein 1 (AP-1) activation, and the integrin αvβ3 mAb, and ASK1, JNK, and p38 inhibitors reduced WISP-1–mediated AP-1 activation. Moreover, WISP-1 and ICAM-1 expression correlated with the tumor stage of patients with OSCC. Our results indicate that WISP-1 enhances the migration of OSCC cells by increasing ICAM-1 expression through the αvβ3 integrin receptor and the ASK1, JNK/p38, and AP-1 signal transduction pathways. Public Library of Science 2013-10-21 /pmc/articles/PMC3804520/ /pubmed/24205072 http://dx.doi.org/10.1371/journal.pone.0078022 Text en © 2013 Chuang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chuang, Jing-Yuan Chang, An-Chen Chiang, I-Ping Tsai, Ming-Hsui Tang, Chih-Hsin Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells |
title | Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells |
title_full | Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells |
title_fullStr | Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells |
title_full_unstemmed | Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells |
title_short | Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells |
title_sort | apoptosis signal-regulating kinase 1 is involved in wisp-1–promoted cell motility in human oral squamous cell carcinoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804520/ https://www.ncbi.nlm.nih.gov/pubmed/24205072 http://dx.doi.org/10.1371/journal.pone.0078022 |
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