Cargando…

Nanobody Mediated Crystallization of an Archeal Mechanosensitive Channel

Mechanosensitive channels (MS) are integral membrane proteins and allow bacteria to survive sudden changes in external osmolarity due to transient opening of their pores. The efflux of cytoplasmic osmolytes reduces the membrane tension and prevents membrane rupture. Therefore these channels serve as...

Descripción completa

Detalles Bibliográficos
Autores principales:	Löw, Christian, Yau, Yin Hoe, Pardon, Els, Jegerschöld, Caroline, Wåhlin, Lisa, Quistgaard, Esben M., Moberg, Per, Geifman-Shochat, Susana, Steyaert, Jan, Nordlund, Pär
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804602/ https://www.ncbi.nlm.nih.gov/pubmed/24205053 http://dx.doi.org/10.1371/journal.pone.0077984

Ejemplares similares

Structural and Biophysical Characterization of the Cytoplasmic Domains of Human BAP29 and BAP31
por: Quistgaard, Esben M., et al.
Publicado: (2013)

Optimisation of Over-Expression in E. coli and Biophysical Characterisation of Human Membrane Protein Synaptogyrin 1
por: Löw, Christian, et al.
Publicado: (2012)

Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody
por: Park, Young-Jun, et al.
Publicado: (2012)

Structural and Biochemical Characterization of Human PR70 in Isolation and in Complex with the Scaffolding Subunit of Protein Phosphatase 2A
por: Dovega, Rebecca, et al.
Publicado: (2014)

Modelling the evolution of the archeal tryptophan synthase
por: Merkl, Rainer
Publicado: (2007)

Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD
por: Quistgaard, Esben M., et al.
Publicado: (2016)

Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody
por: Perez, Camilo, et al.
Publicado: (2017)

Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6∊-FCR3 of the Plasmodium falciparum VAR2CSA protein. Corrigendum
por: Vuchelen, Anneleen, et al.
Publicado: (2013)

Structure determination of a major facilitator peptide transporter: Inward facing PepT(St) from Streptococcus thermophilus crystallized in space group P3(1)21
por: Quistgaard, Esben M., et al.
Publicado: (2017)

An improved yeast surface display platform for the screening of nanobody immune libraries
por: Uchański, Tomasz, et al.
Publicado: (2019)

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
por: Sigoillot, Maud, et al.
Publicado: (2019)

In vivo and in vitro characterizations of melibiose permease (MelB) conformation-dependent nanobodies reveal sugar-binding mechanisms
por: Katsube, Satoshi, et al.
Publicado: (2023)

Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain
por: Wu, Arum, et al.
Publicado: (2023)

Archeal tRNA meets biotechnology: From vaccines to genetic code expansion
por: Ribas de Pouplana, Lluís
Publicado: (2022)

Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies
por: Keown, Jeremy R., et al.
Publicado: (2022)

A novel lipoprotein nanoparticle system for membrane proteins
por: Frauenfeld, Jens, et al.
Publicado: (2016)

Allosteric modulation of the GTPase activity of a bacterial LRRK2 homolog by conformation-specific Nanobodies
por: Leemans, Margaux, et al.
Publicado: (2020)

Nanobody-enabled monitoring of kappa opioid receptor states
por: Che, Tao, et al.
Publicado: (2020)

Identification of Cellular Targets of MicroRNA-181a in HepG2 Cells: A New Approach for Functional Analysis of MicroRNAs
por: Tan, Jane Yi Lin, et al.
Publicado: (2015)

Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN
por: Alonso, Béatrice, et al.
Publicado: (2013)

Structure and Dynamics of an Archeal Monoglyceride Lipase from Palaeococcus ferrophilus as Revealed by Crystallography and In Silico Analysis
por: Labar, Geoffray, et al.
Publicado: (2021)

Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor
por: Gulati, Sahil, et al.
Publicado: (2018)

Tripeptide binding in a proton‐dependent oligopeptide transporter
por: Martinez Molledo, Maria, et al.
Publicado: (2018)

Recognition of RNA duplexes by chemically modified triplex-forming oligonucleotides
por: Zhou, Yuan, et al.
Publicado: (2013)

Bacterial and archeal community composition in hot springs from Indo-Burma region, North-east India
por: Panda, Amrita Kumari, et al.
Publicado: (2016)

Engineering Archeal Surrogate Systems for the Development of Protein–Protein Interaction Inhibitors against Human RAD51
por: Moschetti, Tommaso, et al.
Publicado: (2016)

Characterisation of a synthetic Archeal membrane reveals a possible new adaptation route to extreme conditions
por: Salvador-Castell, Marta, et al.
Publicado: (2021)

Structure of Nanobody Nb23
por: Percipalle, Mathias, et al.
Publicado: (2021)

Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria
por: Breine, A., et al.
Publicado: (2023)

Nanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli
por: Moonens, Kristof, et al.
Publicado: (2014)

Allosteric Nanobodies Reveal the Dynamic Range and Diverse Mechanisms of GPCR Activation
por: Staus, Dean P, et al.
Publicado: (2016)

Crystal structure of human Mediator subunit MED23
por: Monté, Didier, et al.
Publicado: (2018)

Nanobodies as allosteric modulators of Parkinson’s disease–associated LRRK2
por: Singh, Ranjan K., et al.
Publicado: (2022)

High Affinity Human Antibody Fragments to Dengue Virus Non-Structural Protein 3
por: Moreland, Nicole J., et al.
Publicado: (2010)

Structure of a nanobody-stabilized active state of the β(2) adrenoceptor
por: Rasmussen, Søren G. F., et al.
Publicado: (2011)

Sequence and structural analysis of the Asp-box motif and Asp-box beta-propellers; a widespread propeller-type characteristic of the Vps10 domain family and several glycoside hydrolase families
por: Quistgaard, Esben M, et al.
Publicado: (2009)

Multispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter
por: Martinez Molledo, Maria, et al.
Publicado: (2018)

Cryo-EM Structure of an Atypical Proton-Coupled Peptide Transporter: Di- and Tripeptide Permease C
por: Killer, Maxime, et al.
Publicado: (2022)

Comprehensive Analysis and Identification of the Human STIM1 Domains for Structural and Functional Studies
por: How, Jonathan, et al.
Publicado: (2013)

Structural basis of sodium-dependent bile salt uptake into the liver
por: Goutam, Kapil, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6bmdsqng85hcc5n41b5f7i97d3