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A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805516/ https://www.ncbi.nlm.nih.gov/pubmed/24167602 http://dx.doi.org/10.1371/journal.pone.0078142 |
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author | Ferreira, Camila da Silva Abreu, Rodrigo Martins da Silva, Marlone Cunha Ferreira, Aline Siqueira Nasser, Paulo Dominguez Carrilho, Flair José Ono, Suzane Kioko |
author_facet | Ferreira, Camila da Silva Abreu, Rodrigo Martins da Silva, Marlone Cunha Ferreira, Aline Siqueira Nasser, Paulo Dominguez Carrilho, Flair José Ono, Suzane Kioko |
author_sort | Ferreira, Camila da Silva |
collection | PubMed |
description | Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods. |
format | Online Article Text |
id | pubmed-3805516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38055162013-10-28 A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C Ferreira, Camila da Silva Abreu, Rodrigo Martins da Silva, Marlone Cunha Ferreira, Aline Siqueira Nasser, Paulo Dominguez Carrilho, Flair José Ono, Suzane Kioko PLoS One Research Article Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods. Public Library of Science 2013-10-22 /pmc/articles/PMC3805516/ /pubmed/24167602 http://dx.doi.org/10.1371/journal.pone.0078142 Text en © 2013 Ferreira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ferreira, Camila da Silva Abreu, Rodrigo Martins da Silva, Marlone Cunha Ferreira, Aline Siqueira Nasser, Paulo Dominguez Carrilho, Flair José Ono, Suzane Kioko A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
title | A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
title_full | A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
title_fullStr | A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
title_full_unstemmed | A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
title_short | A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
title_sort | fast and cost-effective method for identifying a polymorphism of interleukin 28b related to hepatitis c |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805516/ https://www.ncbi.nlm.nih.gov/pubmed/24167602 http://dx.doi.org/10.1371/journal.pone.0078142 |
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