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A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C

Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979...

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Autores principales: Ferreira, Camila da Silva, Abreu, Rodrigo Martins, da Silva, Marlone Cunha, Ferreira, Aline Siqueira, Nasser, Paulo Dominguez, Carrilho, Flair José, Ono, Suzane Kioko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805516/
https://www.ncbi.nlm.nih.gov/pubmed/24167602
http://dx.doi.org/10.1371/journal.pone.0078142
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author Ferreira, Camila da Silva
Abreu, Rodrigo Martins
da Silva, Marlone Cunha
Ferreira, Aline Siqueira
Nasser, Paulo Dominguez
Carrilho, Flair José
Ono, Suzane Kioko
author_facet Ferreira, Camila da Silva
Abreu, Rodrigo Martins
da Silva, Marlone Cunha
Ferreira, Aline Siqueira
Nasser, Paulo Dominguez
Carrilho, Flair José
Ono, Suzane Kioko
author_sort Ferreira, Camila da Silva
collection PubMed
description Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods.
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spelling pubmed-38055162013-10-28 A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C Ferreira, Camila da Silva Abreu, Rodrigo Martins da Silva, Marlone Cunha Ferreira, Aline Siqueira Nasser, Paulo Dominguez Carrilho, Flair José Ono, Suzane Kioko PLoS One Research Article Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods. Public Library of Science 2013-10-22 /pmc/articles/PMC3805516/ /pubmed/24167602 http://dx.doi.org/10.1371/journal.pone.0078142 Text en © 2013 Ferreira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ferreira, Camila da Silva
Abreu, Rodrigo Martins
da Silva, Marlone Cunha
Ferreira, Aline Siqueira
Nasser, Paulo Dominguez
Carrilho, Flair José
Ono, Suzane Kioko
A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
title A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
title_full A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
title_fullStr A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
title_full_unstemmed A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
title_short A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
title_sort fast and cost-effective method for identifying a polymorphism of interleukin 28b related to hepatitis c
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805516/
https://www.ncbi.nlm.nih.gov/pubmed/24167602
http://dx.doi.org/10.1371/journal.pone.0078142
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