Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

The generation of specific mutant animal models is critical for functional analysis of human genes. The conventional gene targeting approach in embryonic stem cells (ESCs) by homologous recombination is however laborious, slow, expensive, and limited to species with functional ESCs. It is therefore...

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Detalles Bibliográficos
Autores principales: Shen, Bin, Zhang, Xin, Du, Yinan, Wang, Jianying, Gong, Jun, Zhang, Xiaodong, Tate, Peri H., Li, Hongliang, Huang, Xingxu, Zhang, Wensheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805579/
https://www.ncbi.nlm.nih.gov/pubmed/24167580
http://dx.doi.org/10.1371/journal.pone.0077696

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805579/
https://www.ncbi.nlm.nih.gov/pubmed/24167580
http://dx.doi.org/10.1371/journal.pone.0077696

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