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Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measure...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805922/ https://www.ncbi.nlm.nih.gov/pubmed/24155656 http://dx.doi.org/10.7150/ijms.6960 |
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author | Guan, Guo-fang Zhao, Ming Liu, Li-ming Jin, Chun-shun Sun, Kai Zhang, De-jun Yu, Duo-jiao Cao, Hong-wei Lu, Yan -qing Wen, Lian-ji |
author_facet | Guan, Guo-fang Zhao, Ming Liu, Li-ming Jin, Chun-shun Sun, Kai Zhang, De-jun Yu, Duo-jiao Cao, Hong-wei Lu, Yan -qing Wen, Lian-ji |
author_sort | Guan, Guo-fang |
collection | PubMed |
description | An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measured using fluorescence and immunoblotting assays. The attenuated S. typhimurium-mediated Apoptin significantly decreased cytotoxicity and strongly increased cell apoptosis through the activation of caspase-3. The process was mediated by Bax, cytochrome c and caspase-9. A syngeneic nude murine tumour model was used to determine the anti-tumour effects of the recombinant bacteria in vivo. Systemic injection of the recombinant bacteria with and without re-dosing caused significant tumour growth delay and reduced tumour microvessel density, thereby extending host survival. Our findings indicated that the use of recombinant Salmonella typhimurium as an Apoptin expression vector has potential cancer therapeutic benefits. |
format | Online Article Text |
id | pubmed-3805922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-38059222013-10-23 Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer Guan, Guo-fang Zhao, Ming Liu, Li-ming Jin, Chun-shun Sun, Kai Zhang, De-jun Yu, Duo-jiao Cao, Hong-wei Lu, Yan -qing Wen, Lian-ji Int J Med Sci Research Paper An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measured using fluorescence and immunoblotting assays. The attenuated S. typhimurium-mediated Apoptin significantly decreased cytotoxicity and strongly increased cell apoptosis through the activation of caspase-3. The process was mediated by Bax, cytochrome c and caspase-9. A syngeneic nude murine tumour model was used to determine the anti-tumour effects of the recombinant bacteria in vivo. Systemic injection of the recombinant bacteria with and without re-dosing caused significant tumour growth delay and reduced tumour microvessel density, thereby extending host survival. Our findings indicated that the use of recombinant Salmonella typhimurium as an Apoptin expression vector has potential cancer therapeutic benefits. Ivyspring International Publisher 2013-09-21 /pmc/articles/PMC3805922/ /pubmed/24155656 http://dx.doi.org/10.7150/ijms.6960 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Guan, Guo-fang Zhao, Ming Liu, Li-ming Jin, Chun-shun Sun, Kai Zhang, De-jun Yu, Duo-jiao Cao, Hong-wei Lu, Yan -qing Wen, Lian-ji Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer |
title | Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer |
title_full | Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer |
title_fullStr | Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer |
title_full_unstemmed | Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer |
title_short | Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer |
title_sort | salmonella typhimurium mediated delivery of apoptin in human laryngeal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805922/ https://www.ncbi.nlm.nih.gov/pubmed/24155656 http://dx.doi.org/10.7150/ijms.6960 |
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