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Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer

An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measure...

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Autores principales: Guan, Guo-fang, Zhao, Ming, Liu, Li-ming, Jin, Chun-shun, Sun, Kai, Zhang, De-jun, Yu, Duo-jiao, Cao, Hong-wei, Lu, Yan -qing, Wen, Lian-ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805922/
https://www.ncbi.nlm.nih.gov/pubmed/24155656
http://dx.doi.org/10.7150/ijms.6960
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author Guan, Guo-fang
Zhao, Ming
Liu, Li-ming
Jin, Chun-shun
Sun, Kai
Zhang, De-jun
Yu, Duo-jiao
Cao, Hong-wei
Lu, Yan -qing
Wen, Lian-ji
author_facet Guan, Guo-fang
Zhao, Ming
Liu, Li-ming
Jin, Chun-shun
Sun, Kai
Zhang, De-jun
Yu, Duo-jiao
Cao, Hong-wei
Lu, Yan -qing
Wen, Lian-ji
author_sort Guan, Guo-fang
collection PubMed
description An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measured using fluorescence and immunoblotting assays. The attenuated S. typhimurium-mediated Apoptin significantly decreased cytotoxicity and strongly increased cell apoptosis through the activation of caspase-3. The process was mediated by Bax, cytochrome c and caspase-9. A syngeneic nude murine tumour model was used to determine the anti-tumour effects of the recombinant bacteria in vivo. Systemic injection of the recombinant bacteria with and without re-dosing caused significant tumour growth delay and reduced tumour microvessel density, thereby extending host survival. Our findings indicated that the use of recombinant Salmonella typhimurium as an Apoptin expression vector has potential cancer therapeutic benefits.
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spelling pubmed-38059222013-10-23 Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer Guan, Guo-fang Zhao, Ming Liu, Li-ming Jin, Chun-shun Sun, Kai Zhang, De-jun Yu, Duo-jiao Cao, Hong-wei Lu, Yan -qing Wen, Lian-ji Int J Med Sci Research Paper An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measured using fluorescence and immunoblotting assays. The attenuated S. typhimurium-mediated Apoptin significantly decreased cytotoxicity and strongly increased cell apoptosis through the activation of caspase-3. The process was mediated by Bax, cytochrome c and caspase-9. A syngeneic nude murine tumour model was used to determine the anti-tumour effects of the recombinant bacteria in vivo. Systemic injection of the recombinant bacteria with and without re-dosing caused significant tumour growth delay and reduced tumour microvessel density, thereby extending host survival. Our findings indicated that the use of recombinant Salmonella typhimurium as an Apoptin expression vector has potential cancer therapeutic benefits. Ivyspring International Publisher 2013-09-21 /pmc/articles/PMC3805922/ /pubmed/24155656 http://dx.doi.org/10.7150/ijms.6960 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Guan, Guo-fang
Zhao, Ming
Liu, Li-ming
Jin, Chun-shun
Sun, Kai
Zhang, De-jun
Yu, Duo-jiao
Cao, Hong-wei
Lu, Yan -qing
Wen, Lian-ji
Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
title Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
title_full Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
title_fullStr Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
title_full_unstemmed Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
title_short Salmonella typhimurium Mediated Delivery of Apoptin in Human Laryngeal Cancer
title_sort salmonella typhimurium mediated delivery of apoptin in human laryngeal cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805922/
https://www.ncbi.nlm.nih.gov/pubmed/24155656
http://dx.doi.org/10.7150/ijms.6960
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