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Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis
Introduction: Mature circulating endothelial cells (CEC) and circulating endothelial progenitor cells (EPC) have been described in several conditions associated with endothelial injury. Their role in deep vein thrombosis (DVT) has not been previously evaluated. Patients and Methods: In this pilot st...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805926/ https://www.ncbi.nlm.nih.gov/pubmed/24155660 http://dx.doi.org/10.7150/ijms.6887 |
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author | Alessio, Aline M Beltrame, Miriam P Nascimento, Mariane C Flores Vicente, Cristina P de Godoy, Juliana AP Silva, Junia CR Santos Bittar, Luis Fernando Lorand-Metze, Irene de Paula, Erich V Annichino-Bizzacchi, Joyce M |
author_facet | Alessio, Aline M Beltrame, Miriam P Nascimento, Mariane C Flores Vicente, Cristina P de Godoy, Juliana AP Silva, Junia CR Santos Bittar, Luis Fernando Lorand-Metze, Irene de Paula, Erich V Annichino-Bizzacchi, Joyce M |
author_sort | Alessio, Aline M |
collection | PubMed |
description | Introduction: Mature circulating endothelial cells (CEC) and circulating endothelial progenitor cells (EPC) have been described in several conditions associated with endothelial injury. Their role in deep vein thrombosis (DVT) has not been previously evaluated. Patients and Methods: In this pilot study we evaluated the time course of CEC and EPC release after vena cava experimental DVT in mice, using the FeCl3 model. We also evaluated their presence in patients with DVT at different phases of the disease (acute and chronic phase). CEC and EPC were evaluated by Flow Cytometry. Results: In mice, both CEC and EPC were increased 24 hours after DVT induction, peaking 48 hours thereafter. After 72 hours, CEC counts decreased sharply, whereas EPC counts decreased less substantially. In DVT patients we observed a significant increase in CEC counts immediately after DVT compared to healthy individuals. Patients with chronic disease also presented a significant elevation of these cell count. In a subgroup of patients for whom serial samples were available, CEC counts decreased significantly after 9-15 months of the acute event. Conclusions: Our results suggest the participation of these cells in the reparative processes that follows DVT, both at immediate and late time-points. The different kinetics of CEC and EPC release in experimental DVT suggests a heterogeneous role for these cells in the reparative events after DVT. |
format | Online Article Text |
id | pubmed-3805926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-38059262013-10-23 Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis Alessio, Aline M Beltrame, Miriam P Nascimento, Mariane C Flores Vicente, Cristina P de Godoy, Juliana AP Silva, Junia CR Santos Bittar, Luis Fernando Lorand-Metze, Irene de Paula, Erich V Annichino-Bizzacchi, Joyce M Int J Med Sci Research Paper Introduction: Mature circulating endothelial cells (CEC) and circulating endothelial progenitor cells (EPC) have been described in several conditions associated with endothelial injury. Their role in deep vein thrombosis (DVT) has not been previously evaluated. Patients and Methods: In this pilot study we evaluated the time course of CEC and EPC release after vena cava experimental DVT in mice, using the FeCl3 model. We also evaluated their presence in patients with DVT at different phases of the disease (acute and chronic phase). CEC and EPC were evaluated by Flow Cytometry. Results: In mice, both CEC and EPC were increased 24 hours after DVT induction, peaking 48 hours thereafter. After 72 hours, CEC counts decreased sharply, whereas EPC counts decreased less substantially. In DVT patients we observed a significant increase in CEC counts immediately after DVT compared to healthy individuals. Patients with chronic disease also presented a significant elevation of these cell count. In a subgroup of patients for whom serial samples were available, CEC counts decreased significantly after 9-15 months of the acute event. Conclusions: Our results suggest the participation of these cells in the reparative processes that follows DVT, both at immediate and late time-points. The different kinetics of CEC and EPC release in experimental DVT suggests a heterogeneous role for these cells in the reparative events after DVT. Ivyspring International Publisher 2013-10-14 /pmc/articles/PMC3805926/ /pubmed/24155660 http://dx.doi.org/10.7150/ijms.6887 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Alessio, Aline M Beltrame, Miriam P Nascimento, Mariane C Flores Vicente, Cristina P de Godoy, Juliana AP Silva, Junia CR Santos Bittar, Luis Fernando Lorand-Metze, Irene de Paula, Erich V Annichino-Bizzacchi, Joyce M Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis |
title | Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis |
title_full | Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis |
title_fullStr | Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis |
title_full_unstemmed | Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis |
title_short | Circulating Progenitor and Mature Endothelial Cells in Deep Vein Thrombosis |
title_sort | circulating progenitor and mature endothelial cells in deep vein thrombosis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805926/ https://www.ncbi.nlm.nih.gov/pubmed/24155660 http://dx.doi.org/10.7150/ijms.6887 |
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