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N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA

We have recently treated with N(2) gas plasma and achieved inactivation of bacteria. However, the effect of N(2) gas plasma on viruses remains unclear. With the aim of developing this technique, we analyzed the virucidal effect of N(2) gas plasma on influenza virus and its influence on the viral com...

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Autores principales: Sakudo, Akikazu, Shimizu, Naohiro, Imanishi, Yuichiro, Ikuta, Kazuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806126/
https://www.ncbi.nlm.nih.gov/pubmed/24195077
http://dx.doi.org/10.1155/2013/694269
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author Sakudo, Akikazu
Shimizu, Naohiro
Imanishi, Yuichiro
Ikuta, Kazuyoshi
author_facet Sakudo, Akikazu
Shimizu, Naohiro
Imanishi, Yuichiro
Ikuta, Kazuyoshi
author_sort Sakudo, Akikazu
collection PubMed
description We have recently treated with N(2) gas plasma and achieved inactivation of bacteria. However, the effect of N(2) gas plasma on viruses remains unclear. With the aim of developing this technique, we analyzed the virucidal effect of N(2) gas plasma on influenza virus and its influence on the viral components. We treated influenza virus particles with inert N(2) gas plasma (1.5 kpps; kilo pulses per second) produced by a short high-voltage pulse generated from a static induction thyristor power supply. A bioassay using chicken embryonated eggs demonstrated that N(2) gas plasma inactivated influenza virus in allantoic fluid within 5 min. Immunochromatography, enzyme-linked immunosorbent assay, and Coomassie brilliant blue staining showed that N(2) gas plasma treatment of influenza A and B viruses in nasal aspirates and allantoic fluids as well as purified influenza A and B viruses induced degradation of viral proteins including nucleoprotein. Analysis using the polymerase chain reaction suggested that N(2) gas plasma treatment induced changes in the viral RNA genome. Scanning electron microscopy analysis showed that aggregation and fusion of influenza viruses were induced by N(2) gas plasma treatment. We believe these biochemical changes may contribute to the inactivation of influenza viruses by N(2) gas plasma.
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spelling pubmed-38061262013-11-05 N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA Sakudo, Akikazu Shimizu, Naohiro Imanishi, Yuichiro Ikuta, Kazuyoshi Biomed Res Int Research Article We have recently treated with N(2) gas plasma and achieved inactivation of bacteria. However, the effect of N(2) gas plasma on viruses remains unclear. With the aim of developing this technique, we analyzed the virucidal effect of N(2) gas plasma on influenza virus and its influence on the viral components. We treated influenza virus particles with inert N(2) gas plasma (1.5 kpps; kilo pulses per second) produced by a short high-voltage pulse generated from a static induction thyristor power supply. A bioassay using chicken embryonated eggs demonstrated that N(2) gas plasma inactivated influenza virus in allantoic fluid within 5 min. Immunochromatography, enzyme-linked immunosorbent assay, and Coomassie brilliant blue staining showed that N(2) gas plasma treatment of influenza A and B viruses in nasal aspirates and allantoic fluids as well as purified influenza A and B viruses induced degradation of viral proteins including nucleoprotein. Analysis using the polymerase chain reaction suggested that N(2) gas plasma treatment induced changes in the viral RNA genome. Scanning electron microscopy analysis showed that aggregation and fusion of influenza viruses were induced by N(2) gas plasma treatment. We believe these biochemical changes may contribute to the inactivation of influenza viruses by N(2) gas plasma. Hindawi Publishing Corporation 2013 2013-09-30 /pmc/articles/PMC3806126/ /pubmed/24195077 http://dx.doi.org/10.1155/2013/694269 Text en Copyright © 2013 Akikazu Sakudo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sakudo, Akikazu
Shimizu, Naohiro
Imanishi, Yuichiro
Ikuta, Kazuyoshi
N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
title N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
title_full N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
title_fullStr N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
title_full_unstemmed N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
title_short N(2) Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
title_sort n(2) gas plasma inactivates influenza virus by inducing changes in viral surface morphology, protein, and genomic rna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806126/
https://www.ncbi.nlm.nih.gov/pubmed/24195077
http://dx.doi.org/10.1155/2013/694269
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