Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport in Saccharomyces cerevisiae

The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombi...

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Detalles Bibliográficos
Autores principales: Wang, Chengqiang, Shen, Yu, Zhang, Yanyan, Suo, Fan, Hou, Jin, Bao, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806156/
https://www.ncbi.nlm.nih.gov/pubmed/24195072
http://dx.doi.org/10.1155/2013/461204
Descripción
Sumario:The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h(−1), a maximum specific L-arabinose consumption rate of 0.61 g h(−1) g(−1) dry cell weight, and a promising ethanol yield of 0.43 g g(−1) from L-arabinose fermentation.

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