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Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST

LncRNAs (long non-coding RNAs) are thought to play a significant role in cellular homeostasis during development and disease by interacting with CMPs (chromatin-modifying proteins). We recently showed that following transient focal ischemia, the expression of many lncRNAs was altered significantly i...

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Autores principales: Dharap, Ashutosh, Pokrzywa, Courtney, Vemuganti, Raghu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Neurochemistry 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806319/
https://www.ncbi.nlm.nih.gov/pubmed/24063527
http://dx.doi.org/10.1042/AN20130029
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author Dharap, Ashutosh
Pokrzywa, Courtney
Vemuganti, Raghu
author_facet Dharap, Ashutosh
Pokrzywa, Courtney
Vemuganti, Raghu
author_sort Dharap, Ashutosh
collection PubMed
description LncRNAs (long non-coding RNAs) are thought to play a significant role in cellular homeostasis during development and disease by interacting with CMPs (chromatin-modifying proteins). We recently showed that following transient focal ischemia, the expression of many lncRNAs was altered significantly in rat brain. We currently analyzed whether focal ischemia also alters the association of lncRNAs with the CMPs Sin3A and coREST (corepressors of the RE-1 silencing transcription factor). RIP (RNA immunoprecipitation) combined with lncRNA microarray analysis showed that 177 of the 2497 lncRNAs expressed in rat cerebral cortex showed significantly increased binding to either Sin3A or coREST following ischemia compared with sham. Of these, 26 lncRNAs enriched with Sin3A and 11 lncRNAs enriched with coREST were also up-regulated in their expressions after ischemia. A majority of the lncRNAs enriched with these CMPs were intergenic in origin. Evaluation of the expression profiles of corresponding protein-coding genes showed that their expression levels correlate with those of the lncRNAs with which they shared a common locus. This is the first study to show that stroke-induced lncRNAs might associate with CMPs to modulate the post-ischemic epigenetic landscape.
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spelling pubmed-38063192013-10-29 Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST Dharap, Ashutosh Pokrzywa, Courtney Vemuganti, Raghu ASN Neuro Research Article LncRNAs (long non-coding RNAs) are thought to play a significant role in cellular homeostasis during development and disease by interacting with CMPs (chromatin-modifying proteins). We recently showed that following transient focal ischemia, the expression of many lncRNAs was altered significantly in rat brain. We currently analyzed whether focal ischemia also alters the association of lncRNAs with the CMPs Sin3A and coREST (corepressors of the RE-1 silencing transcription factor). RIP (RNA immunoprecipitation) combined with lncRNA microarray analysis showed that 177 of the 2497 lncRNAs expressed in rat cerebral cortex showed significantly increased binding to either Sin3A or coREST following ischemia compared with sham. Of these, 26 lncRNAs enriched with Sin3A and 11 lncRNAs enriched with coREST were also up-regulated in their expressions after ischemia. A majority of the lncRNAs enriched with these CMPs were intergenic in origin. Evaluation of the expression profiles of corresponding protein-coding genes showed that their expression levels correlate with those of the lncRNAs with which they shared a common locus. This is the first study to show that stroke-induced lncRNAs might associate with CMPs to modulate the post-ischemic epigenetic landscape. American Society for Neurochemistry 2013-10-23 /pmc/articles/PMC3806319/ /pubmed/24063527 http://dx.doi.org/10.1042/AN20130029 Text en © 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dharap, Ashutosh
Pokrzywa, Courtney
Vemuganti, Raghu
Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST
title Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST
title_full Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST
title_fullStr Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST
title_full_unstemmed Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST
title_short Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST
title_sort increased binding of stroke-induced long non-coding rnas to the transcriptional corepressors sin3a and corest
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806319/
https://www.ncbi.nlm.nih.gov/pubmed/24063527
http://dx.doi.org/10.1042/AN20130029
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