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Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress

Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the...

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Autores principales: Kern, Florian, Stanika, Ruslan I., Sarg, Bettina, Offterdinger, Martin, Hess, Daniel, Obermair, Gerald J., Lindner, Herbert, Bandtlow, Christine E., Hengst, Ludger, Schweigreiter, Rüdiger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806365/
https://www.ncbi.nlm.nih.gov/pubmed/23909438
http://dx.doi.org/10.1042/BJ20130579
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author Kern, Florian
Stanika, Ruslan I.
Sarg, Bettina
Offterdinger, Martin
Hess, Daniel
Obermair, Gerald J.
Lindner, Herbert
Bandtlow, Christine E.
Hengst, Ludger
Schweigreiter, Rüdiger
author_facet Kern, Florian
Stanika, Ruslan I.
Sarg, Bettina
Offterdinger, Martin
Hess, Daniel
Obermair, Gerald J.
Lindner, Herbert
Bandtlow, Christine E.
Hengst, Ludger
Schweigreiter, Rüdiger
author_sort Kern, Florian
collection PubMed
description Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the CNS. The specificity of Nogo-A resides in its central domain, NiG. We identified Apg-1, a member of the stress-induced Hsp110 (heat-shock protein of 110 kDa) family, as a novel interactor of NiG/Nogo-A. The interaction is selective because Apg-1 interacts with Nogo-A/RTN4-A, but not with RTN1-A, the closest paralogue of Nogo-A. Conversely, Nogo-A binds to Apg-1, but not to Apg-2 or Hsp105, two other members of the Hsp110 family. We characterized the Nogo-A–Apg-1 interaction by affinity precipitation, co-immunoprecipitation and proximity ligation assay, using primary hippocampal neurons derived from Nogo-deficient mice. Under conditions of hypoxic and oxidative stress we found that Nogo-A and Apg-1 were tightly co-regulated in hippocampal neurons. Although both proteins were up-regulated under hypoxic conditions, their expression levels were reduced upon the addition of hydrogen peroxide. Taken together, we suggest that Nogo-A is closely involved in the neuronal response to hypoxic and oxidative stress, an observation that may be of relevance not only in stroke-induced ischaemia, but also in neuroblastoma formation.
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spelling pubmed-38063652013-11-01 Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress Kern, Florian Stanika, Ruslan I. Sarg, Bettina Offterdinger, Martin Hess, Daniel Obermair, Gerald J. Lindner, Herbert Bandtlow, Christine E. Hengst, Ludger Schweigreiter, Rüdiger Biochem J Research Article Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the CNS. The specificity of Nogo-A resides in its central domain, NiG. We identified Apg-1, a member of the stress-induced Hsp110 (heat-shock protein of 110 kDa) family, as a novel interactor of NiG/Nogo-A. The interaction is selective because Apg-1 interacts with Nogo-A/RTN4-A, but not with RTN1-A, the closest paralogue of Nogo-A. Conversely, Nogo-A binds to Apg-1, but not to Apg-2 or Hsp105, two other members of the Hsp110 family. We characterized the Nogo-A–Apg-1 interaction by affinity precipitation, co-immunoprecipitation and proximity ligation assay, using primary hippocampal neurons derived from Nogo-deficient mice. Under conditions of hypoxic and oxidative stress we found that Nogo-A and Apg-1 were tightly co-regulated in hippocampal neurons. Although both proteins were up-regulated under hypoxic conditions, their expression levels were reduced upon the addition of hydrogen peroxide. Taken together, we suggest that Nogo-A is closely involved in the neuronal response to hypoxic and oxidative stress, an observation that may be of relevance not only in stroke-induced ischaemia, but also in neuroblastoma formation. Portland Press Ltd. 2013-09-27 2013-10-15 /pmc/articles/PMC3806365/ /pubmed/23909438 http://dx.doi.org/10.1042/BJ20130579 Text en © 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kern, Florian
Stanika, Ruslan I.
Sarg, Bettina
Offterdinger, Martin
Hess, Daniel
Obermair, Gerald J.
Lindner, Herbert
Bandtlow, Christine E.
Hengst, Ludger
Schweigreiter, Rüdiger
Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
title Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
title_full Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
title_fullStr Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
title_full_unstemmed Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
title_short Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
title_sort nogo-a couples with apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806365/
https://www.ncbi.nlm.nih.gov/pubmed/23909438
http://dx.doi.org/10.1042/BJ20130579
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