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Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806365/ https://www.ncbi.nlm.nih.gov/pubmed/23909438 http://dx.doi.org/10.1042/BJ20130579 |
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author | Kern, Florian Stanika, Ruslan I. Sarg, Bettina Offterdinger, Martin Hess, Daniel Obermair, Gerald J. Lindner, Herbert Bandtlow, Christine E. Hengst, Ludger Schweigreiter, Rüdiger |
author_facet | Kern, Florian Stanika, Ruslan I. Sarg, Bettina Offterdinger, Martin Hess, Daniel Obermair, Gerald J. Lindner, Herbert Bandtlow, Christine E. Hengst, Ludger Schweigreiter, Rüdiger |
author_sort | Kern, Florian |
collection | PubMed |
description | Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the CNS. The specificity of Nogo-A resides in its central domain, NiG. We identified Apg-1, a member of the stress-induced Hsp110 (heat-shock protein of 110 kDa) family, as a novel interactor of NiG/Nogo-A. The interaction is selective because Apg-1 interacts with Nogo-A/RTN4-A, but not with RTN1-A, the closest paralogue of Nogo-A. Conversely, Nogo-A binds to Apg-1, but not to Apg-2 or Hsp105, two other members of the Hsp110 family. We characterized the Nogo-A–Apg-1 interaction by affinity precipitation, co-immunoprecipitation and proximity ligation assay, using primary hippocampal neurons derived from Nogo-deficient mice. Under conditions of hypoxic and oxidative stress we found that Nogo-A and Apg-1 were tightly co-regulated in hippocampal neurons. Although both proteins were up-regulated under hypoxic conditions, their expression levels were reduced upon the addition of hydrogen peroxide. Taken together, we suggest that Nogo-A is closely involved in the neuronal response to hypoxic and oxidative stress, an observation that may be of relevance not only in stroke-induced ischaemia, but also in neuroblastoma formation. |
format | Online Article Text |
id | pubmed-3806365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-38063652013-11-01 Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress Kern, Florian Stanika, Ruslan I. Sarg, Bettina Offterdinger, Martin Hess, Daniel Obermair, Gerald J. Lindner, Herbert Bandtlow, Christine E. Hengst, Ludger Schweigreiter, Rüdiger Biochem J Research Article Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the CNS. The specificity of Nogo-A resides in its central domain, NiG. We identified Apg-1, a member of the stress-induced Hsp110 (heat-shock protein of 110 kDa) family, as a novel interactor of NiG/Nogo-A. The interaction is selective because Apg-1 interacts with Nogo-A/RTN4-A, but not with RTN1-A, the closest paralogue of Nogo-A. Conversely, Nogo-A binds to Apg-1, but not to Apg-2 or Hsp105, two other members of the Hsp110 family. We characterized the Nogo-A–Apg-1 interaction by affinity precipitation, co-immunoprecipitation and proximity ligation assay, using primary hippocampal neurons derived from Nogo-deficient mice. Under conditions of hypoxic and oxidative stress we found that Nogo-A and Apg-1 were tightly co-regulated in hippocampal neurons. Although both proteins were up-regulated under hypoxic conditions, their expression levels were reduced upon the addition of hydrogen peroxide. Taken together, we suggest that Nogo-A is closely involved in the neuronal response to hypoxic and oxidative stress, an observation that may be of relevance not only in stroke-induced ischaemia, but also in neuroblastoma formation. Portland Press Ltd. 2013-09-27 2013-10-15 /pmc/articles/PMC3806365/ /pubmed/23909438 http://dx.doi.org/10.1042/BJ20130579 Text en © 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kern, Florian Stanika, Ruslan I. Sarg, Bettina Offterdinger, Martin Hess, Daniel Obermair, Gerald J. Lindner, Herbert Bandtlow, Christine E. Hengst, Ludger Schweigreiter, Rüdiger Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
title | Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
title_full | Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
title_fullStr | Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
title_full_unstemmed | Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
title_short | Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
title_sort | nogo-a couples with apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806365/ https://www.ncbi.nlm.nih.gov/pubmed/23909438 http://dx.doi.org/10.1042/BJ20130579 |
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