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An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum

Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been de...

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Autores principales: Baskaran, Gunasekaran, Masdor, Noor Azlina, Syed, Mohd Arif, Shukor, Mohd Yunus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806388/
https://www.ncbi.nlm.nih.gov/pubmed/24194687
http://dx.doi.org/10.1155/2013/678356
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author Baskaran, Gunasekaran
Masdor, Noor Azlina
Syed, Mohd Arif
Shukor, Mohd Yunus
author_facet Baskaran, Gunasekaran
Masdor, Noor Azlina
Syed, Mohd Arif
Shukor, Mohd Yunus
author_sort Baskaran, Gunasekaran
collection PubMed
description Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been developed using crude proteases from Coriandrum sativum. In this assay, casein was used as a substrate and Coomassie dye was used to denote the completion of casein hydrolysis. In the absence of inhibitors, casein was hydrolysed and the solution became brown, while in the presence of metal ions such as Hg(2+) and Zn(2+), the hydrolysis of casein was inhibited and the solution remained blue. Both Hg(2+) and Zn(2+) exhibited one-phase binding curve with IC(50) values of 3.217 mg/L and 0.727 mg/L, respectively. The limits of detection (LOD) and limits of quantitation (LOQ) for Hg were 0.241 and 0.802 mg/L, respectively, while the LOD and LOQ for Zn were 0.228 and 0.761 mg/L, respectively. The enzyme exhibited broad pH ranges for activity. The crude proteases extracted from Coriandrum sativum showed good potential for the development of a rapid, sensitive, and economic inhibitive assay for the biomonitoring of Hg(2+) and Zn(2+) in the aquatic environments.
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spelling pubmed-38063882013-11-05 An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum Baskaran, Gunasekaran Masdor, Noor Azlina Syed, Mohd Arif Shukor, Mohd Yunus ScientificWorldJournal Research Article Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been developed using crude proteases from Coriandrum sativum. In this assay, casein was used as a substrate and Coomassie dye was used to denote the completion of casein hydrolysis. In the absence of inhibitors, casein was hydrolysed and the solution became brown, while in the presence of metal ions such as Hg(2+) and Zn(2+), the hydrolysis of casein was inhibited and the solution remained blue. Both Hg(2+) and Zn(2+) exhibited one-phase binding curve with IC(50) values of 3.217 mg/L and 0.727 mg/L, respectively. The limits of detection (LOD) and limits of quantitation (LOQ) for Hg were 0.241 and 0.802 mg/L, respectively, while the LOD and LOQ for Zn were 0.228 and 0.761 mg/L, respectively. The enzyme exhibited broad pH ranges for activity. The crude proteases extracted from Coriandrum sativum showed good potential for the development of a rapid, sensitive, and economic inhibitive assay for the biomonitoring of Hg(2+) and Zn(2+) in the aquatic environments. Hindawi Publishing Corporation 2013-09-30 /pmc/articles/PMC3806388/ /pubmed/24194687 http://dx.doi.org/10.1155/2013/678356 Text en Copyright © 2013 Gunasekaran Baskaran et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Baskaran, Gunasekaran
Masdor, Noor Azlina
Syed, Mohd Arif
Shukor, Mohd Yunus
An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
title An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
title_full An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
title_fullStr An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
title_full_unstemmed An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
title_short An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
title_sort inhibitive enzyme assay to detect mercury and zinc using protease from coriandrum sativum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806388/
https://www.ncbi.nlm.nih.gov/pubmed/24194687
http://dx.doi.org/10.1155/2013/678356
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