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Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma

B7-H3 is a recently discovered member of the B7 superfamily molecules and has been found to play a negative role in T cell responses. In this study, we identified a new B7-H3 isoform that is produced by alternative splicing from the forth intron of B7-H3 and encodes the sB7-H3 protein. Protein seque...

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Detalles Bibliográficos
Autores principales: Chen, Weiwei, Liu, Peixin, Wang, Yedong, Nie, Weimin, Li, Zhiwei, Xu, Wen, Li, Fengyi, Zhou, Zhiping, Zhao, Min, Liu, Henggui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806749/
https://www.ncbi.nlm.nih.gov/pubmed/24194851
http://dx.doi.org/10.1371/journal.pone.0076965
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author Chen, Weiwei
Liu, Peixin
Wang, Yedong
Nie, Weimin
Li, Zhiwei
Xu, Wen
Li, Fengyi
Zhou, Zhiping
Zhao, Min
Liu, Henggui
author_facet Chen, Weiwei
Liu, Peixin
Wang, Yedong
Nie, Weimin
Li, Zhiwei
Xu, Wen
Li, Fengyi
Zhou, Zhiping
Zhao, Min
Liu, Henggui
author_sort Chen, Weiwei
collection PubMed
description B7-H3 is a recently discovered member of the B7 superfamily molecules and has been found to play a negative role in T cell responses. In this study, we identified a new B7-H3 isoform that is produced by alternative splicing from the forth intron of B7-H3 and encodes the sB7-H3 protein. Protein sequence analysis showed that sB7-H3 contains an additional four amino acids, encoded by the intron sequence, at the C-terminus compared to the ectodomain of 2Ig-B7-H3. We further found that this spliced sB7-H3 plays a negative regulatory role in T cell responses and serum sB7-H3 is higher in patients with hepatocellular carcinoma (HCC) than in healthy donors. Furthermore, we found that the expression of the spliced sb7-h3 gene is higher in carcinoma and peritumor tissues than in PBMCs of both healthy controls and patients, indicating that the high level of serum sB7-H3 in patients with HCC is caused by the increased expression of this newly discovered spliced sB7-H3 isoform in carcinoma and peritumor tissues.
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spelling pubmed-38067492013-11-05 Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma Chen, Weiwei Liu, Peixin Wang, Yedong Nie, Weimin Li, Zhiwei Xu, Wen Li, Fengyi Zhou, Zhiping Zhao, Min Liu, Henggui PLoS One Research Article B7-H3 is a recently discovered member of the B7 superfamily molecules and has been found to play a negative role in T cell responses. In this study, we identified a new B7-H3 isoform that is produced by alternative splicing from the forth intron of B7-H3 and encodes the sB7-H3 protein. Protein sequence analysis showed that sB7-H3 contains an additional four amino acids, encoded by the intron sequence, at the C-terminus compared to the ectodomain of 2Ig-B7-H3. We further found that this spliced sB7-H3 plays a negative regulatory role in T cell responses and serum sB7-H3 is higher in patients with hepatocellular carcinoma (HCC) than in healthy donors. Furthermore, we found that the expression of the spliced sb7-h3 gene is higher in carcinoma and peritumor tissues than in PBMCs of both healthy controls and patients, indicating that the high level of serum sB7-H3 in patients with HCC is caused by the increased expression of this newly discovered spliced sB7-H3 isoform in carcinoma and peritumor tissues. Public Library of Science 2013-10-23 /pmc/articles/PMC3806749/ /pubmed/24194851 http://dx.doi.org/10.1371/journal.pone.0076965 Text en © 2013 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Weiwei
Liu, Peixin
Wang, Yedong
Nie, Weimin
Li, Zhiwei
Xu, Wen
Li, Fengyi
Zhou, Zhiping
Zhao, Min
Liu, Henggui
Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma
title Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma
title_full Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma
title_fullStr Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma
title_full_unstemmed Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma
title_short Characterization of a Soluble B7-H3 (sB7-H3) Spliced from the Intron and Analysis of sB7-H3 in the Sera of Patients with Hepatocellular Carcinoma
title_sort characterization of a soluble b7-h3 (sb7-h3) spliced from the intron and analysis of sb7-h3 in the sera of patients with hepatocellular carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806749/
https://www.ncbi.nlm.nih.gov/pubmed/24194851
http://dx.doi.org/10.1371/journal.pone.0076965
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