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Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions
Hydrogen sulfide (H(2)S) is a gasotransmitter and plays an important role in many physiological processes in mammals. Studies of its functions in plants are attracting ever growing interest, for example, its ability to enhance drought resistance in Arabidopsis. A general role of microRNAs (miRNAs) i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806758/ https://www.ncbi.nlm.nih.gov/pubmed/24194857 http://dx.doi.org/10.1371/journal.pone.0077047 |
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author | Shen, Jiejie Xing, Tongji Yuan, Huihong Liu, Zhiqiang Jin, Zhuping Zhang, Liping Pei, Yanxi |
author_facet | Shen, Jiejie Xing, Tongji Yuan, Huihong Liu, Zhiqiang Jin, Zhuping Zhang, Liping Pei, Yanxi |
author_sort | Shen, Jiejie |
collection | PubMed |
description | Hydrogen sulfide (H(2)S) is a gasotransmitter and plays an important role in many physiological processes in mammals. Studies of its functions in plants are attracting ever growing interest, for example, its ability to enhance drought resistance in Arabidopsis. A general role of microRNAs (miRNAs) in plant adaptive responses to drought stress has thereby increased our interest to delve into the possible interplay between H(2)S and miRNAs. Our results showed that treating wild type (WT) Arabidopsis seedlings with polyethylene glycol 8000 (PEG8000) to simulate drought stress caused an increase in production rate of endogenous H(2)S; and a significant transcriptional reformation of relevant miRNAs, which were also triggered by exogenous H(2)S in WT. When lcd mutants (with lower H(2)S production rate than WT) were treated with PEG8000, they showed lower levels of miRNA expression changes than WT. In addition, we detected significant changes in target gene expression of those miRNAs and the corresponding phenotypes in lcd, including less roots, retardation of leaf growth and development and greater superoxide dismutase (SOD) activity under drought stress. We thereby conclude that H(2)S can improve drought resistance through regulating drought associated miRNAs in Arabidopsis. |
format | Online Article Text |
id | pubmed-3806758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38067582013-11-05 Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions Shen, Jiejie Xing, Tongji Yuan, Huihong Liu, Zhiqiang Jin, Zhuping Zhang, Liping Pei, Yanxi PLoS One Research Article Hydrogen sulfide (H(2)S) is a gasotransmitter and plays an important role in many physiological processes in mammals. Studies of its functions in plants are attracting ever growing interest, for example, its ability to enhance drought resistance in Arabidopsis. A general role of microRNAs (miRNAs) in plant adaptive responses to drought stress has thereby increased our interest to delve into the possible interplay between H(2)S and miRNAs. Our results showed that treating wild type (WT) Arabidopsis seedlings with polyethylene glycol 8000 (PEG8000) to simulate drought stress caused an increase in production rate of endogenous H(2)S; and a significant transcriptional reformation of relevant miRNAs, which were also triggered by exogenous H(2)S in WT. When lcd mutants (with lower H(2)S production rate than WT) were treated with PEG8000, they showed lower levels of miRNA expression changes than WT. In addition, we detected significant changes in target gene expression of those miRNAs and the corresponding phenotypes in lcd, including less roots, retardation of leaf growth and development and greater superoxide dismutase (SOD) activity under drought stress. We thereby conclude that H(2)S can improve drought resistance through regulating drought associated miRNAs in Arabidopsis. Public Library of Science 2013-10-23 /pmc/articles/PMC3806758/ /pubmed/24194857 http://dx.doi.org/10.1371/journal.pone.0077047 Text en © 2013 Shen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Shen, Jiejie Xing, Tongji Yuan, Huihong Liu, Zhiqiang Jin, Zhuping Zhang, Liping Pei, Yanxi Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions |
title | Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions |
title_full | Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions |
title_fullStr | Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions |
title_full_unstemmed | Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions |
title_short | Hydrogen Sulfide Improves Drought Tolerance in Arabidopsis thaliana by MicroRNA Expressions |
title_sort | hydrogen sulfide improves drought tolerance in arabidopsis thaliana by microrna expressions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806758/ https://www.ncbi.nlm.nih.gov/pubmed/24194857 http://dx.doi.org/10.1371/journal.pone.0077047 |
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