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BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate

Bone morphogenic proteins (BMPs) are critical for both chondrogenesis and osteogenesis. Previous studies reported that embryos deficient in Bmp receptor (Bmpr)1a or Bmpr1b in cartilage display subtle skeletal defects; however, double mutant embryos develop severe skeletal defects, suggesting a funct...

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Autores principales: Jing, Junjun, Ren, Yinshi, Zong, Zhaowen, Liu, Chuanju, Kamiya, Nobuhiro, Mishina, Yuji, Liu, Ying, Zhou, Xuedong, Feng, Jian Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807016/
https://www.ncbi.nlm.nih.gov/pubmed/24163588
http://dx.doi.org/10.7150/ijbs.7508
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author Jing, Junjun
Ren, Yinshi
Zong, Zhaowen
Liu, Chuanju
Kamiya, Nobuhiro
Mishina, Yuji
Liu, Ying
Zhou, Xuedong
Feng, Jian Q.
author_facet Jing, Junjun
Ren, Yinshi
Zong, Zhaowen
Liu, Chuanju
Kamiya, Nobuhiro
Mishina, Yuji
Liu, Ying
Zhou, Xuedong
Feng, Jian Q.
author_sort Jing, Junjun
collection PubMed
description Bone morphogenic proteins (BMPs) are critical for both chondrogenesis and osteogenesis. Previous studies reported that embryos deficient in Bmp receptor (Bmpr)1a or Bmpr1b in cartilage display subtle skeletal defects; however, double mutant embryos develop severe skeletal defects, suggesting a functional redundancy that is essential for early chondrogenesis. In this study, we examined the postnatal role of Bmpr1a in cartilage. In the Bmpr1a conditional knockout (cKO, a cross between Bmpr1a flox and aggrecan-CreER(T2) induced by a one-time-tamoxifen injection at birth and harvested at ages of 2, 4, 8 and 20 weeks), there was essentially no long bone growth with little expression of cartilage markers such as SOX9, IHH and glycoproteins. Unexpectedly, the null growth plate was replaced by bone-like tissues, supporting the notions that the progenitor cells in the growth plate, which normally form cartilage, can form other tissues such as bone and fibrous; and that BMPR1A determines the cell fate. A working hypothesis is proposed to explain the vital role of BMPR1A in postnatal chondrogenesis.
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spelling pubmed-38070162013-10-25 BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate Jing, Junjun Ren, Yinshi Zong, Zhaowen Liu, Chuanju Kamiya, Nobuhiro Mishina, Yuji Liu, Ying Zhou, Xuedong Feng, Jian Q. Int J Biol Sci Research Paper Bone morphogenic proteins (BMPs) are critical for both chondrogenesis and osteogenesis. Previous studies reported that embryos deficient in Bmp receptor (Bmpr)1a or Bmpr1b in cartilage display subtle skeletal defects; however, double mutant embryos develop severe skeletal defects, suggesting a functional redundancy that is essential for early chondrogenesis. In this study, we examined the postnatal role of Bmpr1a in cartilage. In the Bmpr1a conditional knockout (cKO, a cross between Bmpr1a flox and aggrecan-CreER(T2) induced by a one-time-tamoxifen injection at birth and harvested at ages of 2, 4, 8 and 20 weeks), there was essentially no long bone growth with little expression of cartilage markers such as SOX9, IHH and glycoproteins. Unexpectedly, the null growth plate was replaced by bone-like tissues, supporting the notions that the progenitor cells in the growth plate, which normally form cartilage, can form other tissues such as bone and fibrous; and that BMPR1A determines the cell fate. A working hypothesis is proposed to explain the vital role of BMPR1A in postnatal chondrogenesis. Ivyspring International Publisher 2013-09-18 /pmc/articles/PMC3807016/ /pubmed/24163588 http://dx.doi.org/10.7150/ijbs.7508 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Jing, Junjun
Ren, Yinshi
Zong, Zhaowen
Liu, Chuanju
Kamiya, Nobuhiro
Mishina, Yuji
Liu, Ying
Zhou, Xuedong
Feng, Jian Q.
BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate
title BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate
title_full BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate
title_fullStr BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate
title_full_unstemmed BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate
title_short BMP Receptor 1A Determines the Cell Fate of the Postnatal Growth Plate
title_sort bmp receptor 1a determines the cell fate of the postnatal growth plate
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807016/
https://www.ncbi.nlm.nih.gov/pubmed/24163588
http://dx.doi.org/10.7150/ijbs.7508
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