Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

The mature cDNA of endochitinase from Trichoderma viride sp. was optimised based on the codon bias of Pichia pastoris GS115 and synthesised by successive PCR; the sequence was then transformed into P. pastoris GS115 via electroporation. The transformant with the fastest growth rate on YPD plates con...

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Detalles Bibliográficos
Autores principales: Yu, Ping, Yan, Yuan, Gu, Qing, Wang, Xiangyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807108/
https://www.ncbi.nlm.nih.gov/pubmed/24154717
http://dx.doi.org/10.1038/srep03043

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807108/
https://www.ncbi.nlm.nih.gov/pubmed/24154717
http://dx.doi.org/10.1038/srep03043

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