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Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris
The mature cDNA of endochitinase from Trichoderma viride sp. was optimised based on the codon bias of Pichia pastoris GS115 and synthesised by successive PCR; the sequence was then transformed into P. pastoris GS115 via electroporation. The transformant with the fastest growth rate on YPD plates con...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807108/ https://www.ncbi.nlm.nih.gov/pubmed/24154717 http://dx.doi.org/10.1038/srep03043 |