A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor th...

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Detalles Bibliográficos
Autores principales: Leung, Ka-Ho, Lu, Lihua, Wang, Modi, Mak, Tsun-Yin, Chan, Daniel Shiu-Hin, Tang, Fung-Kit, Leung, Chung-Hang, Kwan, Hiu-Yee, Yu, Zhiling, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808368/
https://www.ncbi.nlm.nih.gov/pubmed/24204723
http://dx.doi.org/10.1371/journal.pone.0077021
Descripción
Sumario:We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.

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