A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor th...

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Autores principales: Leung, Ka-Ho, Lu, Lihua, Wang, Modi, Mak, Tsun-Yin, Chan, Daniel Shiu-Hin, Tang, Fung-Kit, Leung, Chung-Hang, Kwan, Hiu-Yee, Yu, Zhiling, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808368/
https://www.ncbi.nlm.nih.gov/pubmed/24204723
http://dx.doi.org/10.1371/journal.pone.0077021
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author Leung, Ka-Ho
Lu, Lihua
Wang, Modi
Mak, Tsun-Yin
Chan, Daniel Shiu-Hin
Tang, Fung-Kit
Leung, Chung-Hang
Kwan, Hiu-Yee
Yu, Zhiling
Ma, Dik-Lung
author_facet Leung, Ka-Ho
Lu, Lihua
Wang, Modi
Mak, Tsun-Yin
Chan, Daniel Shiu-Hin
Tang, Fung-Kit
Leung, Chung-Hang
Kwan, Hiu-Yee
Yu, Zhiling
Ma, Dik-Lung
author_sort Leung, Ka-Ho
collection PubMed
description We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.
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spelling pubmed-38083682013-11-07 A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex Leung, Ka-Ho Lu, Lihua Wang, Modi Mak, Tsun-Yin Chan, Daniel Shiu-Hin Tang, Fung-Kit Leung, Chung-Hang Kwan, Hiu-Yee Yu, Zhiling Ma, Dik-Lung PLoS One Research Article We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM. Public Library of Science 2013-10-25 /pmc/articles/PMC3808368/ /pubmed/24204723 http://dx.doi.org/10.1371/journal.pone.0077021 Text en © 2013 Leung et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Leung, Ka-Ho
Lu, Lihua
Wang, Modi
Mak, Tsun-Yin
Chan, Daniel Shiu-Hin
Tang, Fung-Kit
Leung, Chung-Hang
Kwan, Hiu-Yee
Yu, Zhiling
Ma, Dik-Lung
A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
title A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
title_full A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
title_fullStr A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
title_full_unstemmed A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
title_short A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
title_sort label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(iii) complex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808368/
https://www.ncbi.nlm.nih.gov/pubmed/24204723
http://dx.doi.org/10.1371/journal.pone.0077021
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