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Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates
Background: Brucellosis is an endemic zoonosis in Syria, affecting large numbers of animals. There are an increasing number of cases in humans. Brucella is a facultative intracellular pathogen, a small, non-motile, Gram-negative coccobacillus, which causes abortion in domestic animals and a febrile...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shiraz University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808949/ https://www.ncbi.nlm.nih.gov/pubmed/24174696 |
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author | Al-Mariri, Ayman Safi, Mazen |
author_facet | Al-Mariri, Ayman Safi, Mazen |
author_sort | Al-Mariri, Ayman |
collection | PubMed |
description | Background: Brucellosis is an endemic zoonosis in Syria, affecting large numbers of animals. There are an increasing number of cases in humans. Brucella is a facultative intracellular pathogen, a small, non-motile, Gram-negative coccobacillus, which causes abortion in domestic animals and a febrile illness in humans. Methods: One hundred isolates collected from different Syrian regions were confirmed to be Brucella melitensis by biochemical tests. The minimum inhibitory concentration (MIC) of 6 antibiotics, alone and in combination, was determined at pH 7.0 and pH 5.0. Results: Ciprofloxacin and sparfloxacin were the most effective antibiotics tested at either pH value. In contrast, rifampicin had low activity and streptomycin was ineffective at either pH value. A combination of rifampicin-doxycycline revealed the highest synergistic activity at both test pH values (against 19/24 and 17/24 isolates, respectively) in vitro. Antagonistic activities were observed using a ciprofloxacin-streptomycin combination (against 9/24 and 13/24 isolates, respectively) as well as a ciprofloxacin-tetracycline combination (against 6/24 and 9/24 isolates, respectively). No differences were observed at both test pH values, when combining a Quinolone with rifampicin or doxycycline. Conclusion: Combination of a Quinolone with doxycycline demonstrated good in vitro activity against B. melitensis. Further in vivo studies are necessary to support this suggestion. |
format | Online Article Text |
id | pubmed-3808949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Shiraz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-38089492013-10-30 Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates Al-Mariri, Ayman Safi, Mazen Iran J Med Sci Original Article Background: Brucellosis is an endemic zoonosis in Syria, affecting large numbers of animals. There are an increasing number of cases in humans. Brucella is a facultative intracellular pathogen, a small, non-motile, Gram-negative coccobacillus, which causes abortion in domestic animals and a febrile illness in humans. Methods: One hundred isolates collected from different Syrian regions were confirmed to be Brucella melitensis by biochemical tests. The minimum inhibitory concentration (MIC) of 6 antibiotics, alone and in combination, was determined at pH 7.0 and pH 5.0. Results: Ciprofloxacin and sparfloxacin were the most effective antibiotics tested at either pH value. In contrast, rifampicin had low activity and streptomycin was ineffective at either pH value. A combination of rifampicin-doxycycline revealed the highest synergistic activity at both test pH values (against 19/24 and 17/24 isolates, respectively) in vitro. Antagonistic activities were observed using a ciprofloxacin-streptomycin combination (against 9/24 and 13/24 isolates, respectively) as well as a ciprofloxacin-tetracycline combination (against 6/24 and 9/24 isolates, respectively). No differences were observed at both test pH values, when combining a Quinolone with rifampicin or doxycycline. Conclusion: Combination of a Quinolone with doxycycline demonstrated good in vitro activity against B. melitensis. Further in vivo studies are necessary to support this suggestion. Shiraz University of Medical Sciences 2013-09 /pmc/articles/PMC3808949/ /pubmed/24174696 Text en © 2013: Iranian Journal of Medical Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Al-Mariri, Ayman Safi, Mazen Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates |
title | Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates |
title_full | Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates |
title_fullStr | Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates |
title_full_unstemmed | Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates |
title_short | Effect of Medium pH on Antibiotic Activity against Syrian Brucella spp. Isolates |
title_sort | effect of medium ph on antibiotic activity against syrian brucella spp. isolates |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808949/ https://www.ncbi.nlm.nih.gov/pubmed/24174696 |
work_keys_str_mv | AT almaririayman effectofmediumphonantibioticactivityagainstsyrianbrucellasppisolates AT safimazen effectofmediumphonantibioticactivityagainstsyrianbrucellasppisolates |