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Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis

OBJECTIVE: In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF) in a rat model of diabetes mellitus (DM) and periodontitis (PD). Additionally, we evaluated alveolar bone loss and the histopathological response associ...

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Autores principales: Jiang, Zhu-Ling, Cui, Yu-Qiong, Gao, Rui, Li, Ying, Fu, Zhao-Chen, Zhang, Bin, Guan, Cheng-Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3809972/
https://www.ncbi.nlm.nih.gov/pubmed/23478270
http://dx.doi.org/10.3233/DMA-130974
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author Jiang, Zhu-Ling
Cui, Yu-Qiong
Gao, Rui
Li, Ying
Fu, Zhao-Chen
Zhang, Bin
Guan, Cheng-Chao
author_facet Jiang, Zhu-Ling
Cui, Yu-Qiong
Gao, Rui
Li, Ying
Fu, Zhao-Chen
Zhang, Bin
Guan, Cheng-Chao
author_sort Jiang, Zhu-Ling
collection PubMed
description OBJECTIVE: In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF) in a rat model of diabetes mellitus (DM) and periodontitis (PD). Additionally, we evaluated alveolar bone loss and the histopathological response associated with experimental diabetes mellitus and experimental periodontitis. METHODS: DM and PD were induced together in 15 rats (group 1) by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats (group 2), diabetes alone was produced by streptozotocin injection in 15 rats (group 3), and fifteen systemically and periodontally healthy rats were used as controls (group 4). The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Periodontal destruction was assessed by measuring the alveolar bone loss. Periodontal inflammation was quantified by histopathological grading in H&E stained samples. RESULTS: Higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology were found in group 1 compared with group 2, group 3 and group 4 (p < 0.05). The quantities of TNF-α, IL1-β and LPS, the amount of alveolar bone loss and the severity of the histopathological finding were greater in group 2 than group 3 and group 4 (p < 0.05). Group 3 demonstrated higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology than group 4 (p < 0.05). Statistically significant differences were noted between all of the groups. CONCLUSIONS: These data indicate that DM may lead to enhanced TNF-α, IL1-β and LPS production in the periodontal tissues. The resorption values of alveolar bone and the histological inflammation were more severe in rats with periodontitis and diabetes mellitus than in those with periodontitis alone, diabetes mellitus alone and control rats. Our findings are consistent with the hypothesis that hyperglycemia contributes to the heightened inflammatory response associated with periodontitis.
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spelling pubmed-38099722013-12-02 Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis Jiang, Zhu-Ling Cui, Yu-Qiong Gao, Rui Li, Ying Fu, Zhao-Chen Zhang, Bin Guan, Cheng-Chao Dis Markers Other OBJECTIVE: In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF) in a rat model of diabetes mellitus (DM) and periodontitis (PD). Additionally, we evaluated alveolar bone loss and the histopathological response associated with experimental diabetes mellitus and experimental periodontitis. METHODS: DM and PD were induced together in 15 rats (group 1) by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats (group 2), diabetes alone was produced by streptozotocin injection in 15 rats (group 3), and fifteen systemically and periodontally healthy rats were used as controls (group 4). The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Periodontal destruction was assessed by measuring the alveolar bone loss. Periodontal inflammation was quantified by histopathological grading in H&E stained samples. RESULTS: Higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology were found in group 1 compared with group 2, group 3 and group 4 (p < 0.05). The quantities of TNF-α, IL1-β and LPS, the amount of alveolar bone loss and the severity of the histopathological finding were greater in group 2 than group 3 and group 4 (p < 0.05). Group 3 demonstrated higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology than group 4 (p < 0.05). Statistically significant differences were noted between all of the groups. CONCLUSIONS: These data indicate that DM may lead to enhanced TNF-α, IL1-β and LPS production in the periodontal tissues. The resorption values of alveolar bone and the histological inflammation were more severe in rats with periodontitis and diabetes mellitus than in those with periodontitis alone, diabetes mellitus alone and control rats. Our findings are consistent with the hypothesis that hyperglycemia contributes to the heightened inflammatory response associated with periodontitis. IOS Press 2013 2013-05-21 /pmc/articles/PMC3809972/ /pubmed/23478270 http://dx.doi.org/10.3233/DMA-130974 Text en Copyright © 2013 Hindawi Publishing Corporation.
spellingShingle Other
Jiang, Zhu-Ling
Cui, Yu-Qiong
Gao, Rui
Li, Ying
Fu, Zhao-Chen
Zhang, Bin
Guan, Cheng-Chao
Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis
title Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis
title_full Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis
title_fullStr Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis
title_full_unstemmed Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis
title_short Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis
title_sort study of tnf-α, il-1β and lps levels in the gingival crevicular fluid of a rat model of diabetes mellitus and periodontitis
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3809972/
https://www.ncbi.nlm.nih.gov/pubmed/23478270
http://dx.doi.org/10.3233/DMA-130974
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