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Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles
In vertebrates, the majority of mRNAs that encode secreted, membrane-bound or mitochondrial proteins contain RNA elements that activate an alternative mRNA nuclear export (ALREX) pathway. Here we demonstrate that mRNAs containing ALREX-promoting elements are trafficked through nuclear speckles. Alth...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810340/ https://www.ncbi.nlm.nih.gov/pubmed/23934081 http://dx.doi.org/10.4161/nucl.26052 |
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author | Akef, Abdalla Zhang, Hui Masuda, Seiji Palazzo, Alexander F |
author_facet | Akef, Abdalla Zhang, Hui Masuda, Seiji Palazzo, Alexander F |
author_sort | Akef, Abdalla |
collection | PubMed |
description | In vertebrates, the majority of mRNAs that encode secreted, membrane-bound or mitochondrial proteins contain RNA elements that activate an alternative mRNA nuclear export (ALREX) pathway. Here we demonstrate that mRNAs containing ALREX-promoting elements are trafficked through nuclear speckles. Although ALREX-promoting elements enhance nuclear speckle localization, additional features within the mRNA largely drive this process. Depletion of two TREX-associated RNA helicases, UAP56 and its paralog URH49, or inhibition of the TREX-associated nuclear transport factor, TAP, not only inhibits ALREX, but also appears to trap these mRNAs in nuclear speckles. mRNAs that contain ALREX-promoting elements associate with UAP56 in vivo. Finally, we demonstrate that mRNAs lacking a poly(A)-tail are not efficiently exported by the ALREX pathway and show enhanced association with nuclear speckles. Our data suggest that within the speckle, ALREX-promoting elements, in conjunction with the poly(A)-tail, likely stimulate UAP56/URH49 and TAP dependent steps that lead to the eventual egress of the export-competent mRNP from these structures. |
format | Online Article Text |
id | pubmed-3810340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-38103402013-10-30 Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles Akef, Abdalla Zhang, Hui Masuda, Seiji Palazzo, Alexander F Nucleus Research Paper In vertebrates, the majority of mRNAs that encode secreted, membrane-bound or mitochondrial proteins contain RNA elements that activate an alternative mRNA nuclear export (ALREX) pathway. Here we demonstrate that mRNAs containing ALREX-promoting elements are trafficked through nuclear speckles. Although ALREX-promoting elements enhance nuclear speckle localization, additional features within the mRNA largely drive this process. Depletion of two TREX-associated RNA helicases, UAP56 and its paralog URH49, or inhibition of the TREX-associated nuclear transport factor, TAP, not only inhibits ALREX, but also appears to trap these mRNAs in nuclear speckles. mRNAs that contain ALREX-promoting elements associate with UAP56 in vivo. Finally, we demonstrate that mRNAs lacking a poly(A)-tail are not efficiently exported by the ALREX pathway and show enhanced association with nuclear speckles. Our data suggest that within the speckle, ALREX-promoting elements, in conjunction with the poly(A)-tail, likely stimulate UAP56/URH49 and TAP dependent steps that lead to the eventual egress of the export-competent mRNP from these structures. Landes Bioscience 2013-07-01 2013-08-08 /pmc/articles/PMC3810340/ /pubmed/23934081 http://dx.doi.org/10.4161/nucl.26052 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Research Paper Akef, Abdalla Zhang, Hui Masuda, Seiji Palazzo, Alexander F Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles |
title | Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles |
title_full | Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles |
title_fullStr | Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles |
title_full_unstemmed | Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles |
title_short | Trafficking of mRNAs containing ALREX-promoting elements through nuclear speckles |
title_sort | trafficking of mrnas containing alrex-promoting elements through nuclear speckles |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810340/ https://www.ncbi.nlm.nih.gov/pubmed/23934081 http://dx.doi.org/10.4161/nucl.26052 |
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