Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus

Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment...

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Detalles Bibliográficos
Autores principales: Qiu, Chao, Huang, Yang, Zhang, Anli, Tian, Di, Wan, Yanmin, Zhang, Xiaoling, Zhang, Wanju, Zhang, Zhiyong, Yuan, Zhenghong, Hu, Yunwen, Zhang, Xiaoyan, Xu, Jianqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2013
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810762/
https://www.ncbi.nlm.nih.gov/pubmed/24047684
http://dx.doi.org/10.3201/eid1910.130728
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author Qiu, Chao
Huang, Yang
Zhang, Anli
Tian, Di
Wan, Yanmin
Zhang, Xiaoling
Zhang, Wanju
Zhang, Zhiyong
Yuan, Zhenghong
Hu, Yunwen
Zhang, Xiaoyan
Xu, Jianqing
author_facet Qiu, Chao
Huang, Yang
Zhang, Anli
Tian, Di
Wan, Yanmin
Zhang, Xiaoling
Zhang, Wanju
Zhang, Zhiyong
Yuan, Zhenghong
Hu, Yunwen
Zhang, Xiaoyan
Xu, Jianqing
author_sort Qiu, Chao
collection PubMed
description Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment using subtype H7N9–infected samples and controls.
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spelling pubmed-38107622013-11-05 Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus Qiu, Chao Huang, Yang Zhang, Anli Tian, Di Wan, Yanmin Zhang, Xiaoling Zhang, Wanju Zhang, Zhiyong Yuan, Zhenghong Hu, Yunwen Zhang, Xiaoyan Xu, Jianqing Emerg Infect Dis Dispatch Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment using subtype H7N9–infected samples and controls. Centers for Disease Control and Prevention 2013-10 /pmc/articles/PMC3810762/ /pubmed/24047684 http://dx.doi.org/10.3201/eid1910.130728 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Qiu, Chao
Huang, Yang
Zhang, Anli
Tian, Di
Wan, Yanmin
Zhang, Xiaoling
Zhang, Wanju
Zhang, Zhiyong
Yuan, Zhenghong
Hu, Yunwen
Zhang, Xiaoyan
Xu, Jianqing
Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_full Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_fullStr Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_full_unstemmed Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_short Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_sort safe pseudovirus-based assay for neutralization antibodies against influenza a(h7n9) virus
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810762/
https://www.ncbi.nlm.nih.gov/pubmed/24047684
http://dx.doi.org/10.3201/eid1910.130728
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