Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity

Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allow...

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Autores principales: Russell, B., Malleret, B., Suwanarusk, R., Anthony, C., Kanlaya, S., Lau, Y. L., Woodrow, C. J., Nosten, F., Renia, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3811473/
https://www.ncbi.nlm.nih.gov/pubmed/23877705
http://dx.doi.org/10.1128/AAC.00682-13
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author Russell, B.
Malleret, B.
Suwanarusk, R.
Anthony, C.
Kanlaya, S.
Lau, Y. L.
Woodrow, C. J.
Nosten, F.
Renia, L.
author_facet Russell, B.
Malleret, B.
Suwanarusk, R.
Anthony, C.
Kanlaya, S.
Lau, Y. L.
Woodrow, C. J.
Nosten, F.
Renia, L.
author_sort Russell, B.
collection PubMed
description Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated Plasmodium vivax and Plasmodium falciparum.
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spelling pubmed-38114732013-11-14 Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity Russell, B. Malleret, B. Suwanarusk, R. Anthony, C. Kanlaya, S. Lau, Y. L. Woodrow, C. J. Nosten, F. Renia, L. Antimicrob Agents Chemother Susceptibility Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated Plasmodium vivax and Plasmodium falciparum. American Society for Microbiology 2013-10 /pmc/articles/PMC3811473/ /pubmed/23877705 http://dx.doi.org/10.1128/AAC.00682-13 Text en Copyright © 2013 Russell et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Susceptibility
Russell, B.
Malleret, B.
Suwanarusk, R.
Anthony, C.
Kanlaya, S.
Lau, Y. L.
Woodrow, C. J.
Nosten, F.
Renia, L.
Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity
title Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity
title_full Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity
title_fullStr Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity
title_full_unstemmed Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity
title_short Field-Based Flow Cytometry for Ex Vivo Characterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity
title_sort field-based flow cytometry for ex vivo characterization of plasmodium vivax and p. falciparum antimalarial sensitivity
topic Susceptibility
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3811473/
https://www.ncbi.nlm.nih.gov/pubmed/23877705
http://dx.doi.org/10.1128/AAC.00682-13
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