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Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella

Legionella pneumophila, a facultative intracellular bacterium, is the causative agent of legionellosis. In the environment this pathogenic bacterium colonizes the biofilms as well as amoebae, which provide a rich environment for the replication of Legionella. When seeded on pre-formed biofilms, L. p...

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Autores principales: Bigot, Renaud, Bertaux, Joanne, Frere, Jacques, Berjeaud, Jean-Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812023/
https://www.ncbi.nlm.nih.gov/pubmed/24205008
http://dx.doi.org/10.1371/journal.pone.0077875
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author Bigot, Renaud
Bertaux, Joanne
Frere, Jacques
Berjeaud, Jean-Marc
author_facet Bigot, Renaud
Bertaux, Joanne
Frere, Jacques
Berjeaud, Jean-Marc
author_sort Bigot, Renaud
collection PubMed
description Legionella pneumophila, a facultative intracellular bacterium, is the causative agent of legionellosis. In the environment this pathogenic bacterium colonizes the biofilms as well as amoebae, which provide a rich environment for the replication of Legionella. When seeded on pre-formed biofilms, L. pneumophila was able to establish and survive and was only found at the surface of the biofilms. Different phenotypes were observed when the L. pneumophila, used to implement pre-formed biofilms or to form mono-species biofilms, were cultivated in a laboratory culture broth or had grown intracellulary within the amoeba. Indeed, the bacteria, which developed within the amoeba, formed clusters when deposited on a solid surface. Moreover, our results demonstrate that multiplication inside the amoeba increased the capacity of L. pneumophila to produce polysaccharides and therefore enhanced its capacity to establish biofilms. Finally, it was shown that the clusters formed by L. pneumophila were probably related to the secretion of a chemotaxis molecular agent.
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spelling pubmed-38120232013-11-07 Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella Bigot, Renaud Bertaux, Joanne Frere, Jacques Berjeaud, Jean-Marc PLoS One Research Article Legionella pneumophila, a facultative intracellular bacterium, is the causative agent of legionellosis. In the environment this pathogenic bacterium colonizes the biofilms as well as amoebae, which provide a rich environment for the replication of Legionella. When seeded on pre-formed biofilms, L. pneumophila was able to establish and survive and was only found at the surface of the biofilms. Different phenotypes were observed when the L. pneumophila, used to implement pre-formed biofilms or to form mono-species biofilms, were cultivated in a laboratory culture broth or had grown intracellulary within the amoeba. Indeed, the bacteria, which developed within the amoeba, formed clusters when deposited on a solid surface. Moreover, our results demonstrate that multiplication inside the amoeba increased the capacity of L. pneumophila to produce polysaccharides and therefore enhanced its capacity to establish biofilms. Finally, it was shown that the clusters formed by L. pneumophila were probably related to the secretion of a chemotaxis molecular agent. Public Library of Science 2013-10-24 /pmc/articles/PMC3812023/ /pubmed/24205008 http://dx.doi.org/10.1371/journal.pone.0077875 Text en © 2013 Bigot et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bigot, Renaud
Bertaux, Joanne
Frere, Jacques
Berjeaud, Jean-Marc
Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
title Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
title_full Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
title_fullStr Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
title_full_unstemmed Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
title_short Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
title_sort intra-amoeba multiplication induces chemotaxis and biofilm colonization and formation for legionella
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812023/
https://www.ncbi.nlm.nih.gov/pubmed/24205008
http://dx.doi.org/10.1371/journal.pone.0077875
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