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Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812964/ https://www.ncbi.nlm.nih.gov/pubmed/24145165 http://dx.doi.org/10.1083/jcb.201304063 |
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author | Oddoux, Sarah Zaal, Kristien J. Tate, Victoria Kenea, Aster Nandkeolyar, Shuktika A. Reid, Ericka Liu, Wenhua Ralston, Evelyn |
author_facet | Oddoux, Sarah Zaal, Kristien J. Tate, Victoria Kenea, Aster Nandkeolyar, Shuktika A. Reid, Ericka Liu, Wenhua Ralston, Evelyn |
author_sort | Oddoux, Sarah |
collection | PubMed |
description | Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital, and superresolution microscopy, we find that muscle MTs are dynamic, growing at the typical speed of ∼9 µm/min, and forming small bundles that build a durable network. We also show that static Golgi elements, associated with the MT-organizing center proteins γ-tubulin and pericentrin, are major sites of muscle MT nucleation, in addition to the previously identified sites (i.e., nuclear membranes). These data give us a framework for understanding how muscle MTs organize and how they contribute to the pathology of muscle diseases such as Duchenne muscular dystrophy. |
format | Online Article Text |
id | pubmed-3812964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-38129642014-04-28 Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements Oddoux, Sarah Zaal, Kristien J. Tate, Victoria Kenea, Aster Nandkeolyar, Shuktika A. Reid, Ericka Liu, Wenhua Ralston, Evelyn J Cell Biol Research Articles Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital, and superresolution microscopy, we find that muscle MTs are dynamic, growing at the typical speed of ∼9 µm/min, and forming small bundles that build a durable network. We also show that static Golgi elements, associated with the MT-organizing center proteins γ-tubulin and pericentrin, are major sites of muscle MT nucleation, in addition to the previously identified sites (i.e., nuclear membranes). These data give us a framework for understanding how muscle MTs organize and how they contribute to the pathology of muscle diseases such as Duchenne muscular dystrophy. The Rockefeller University Press 2013-10-28 /pmc/articles/PMC3812964/ /pubmed/24145165 http://dx.doi.org/10.1083/jcb.201304063 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Oddoux, Sarah Zaal, Kristien J. Tate, Victoria Kenea, Aster Nandkeolyar, Shuktika A. Reid, Ericka Liu, Wenhua Ralston, Evelyn Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements |
title | Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements |
title_full | Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements |
title_fullStr | Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements |
title_full_unstemmed | Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements |
title_short | Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements |
title_sort | microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at golgi elements |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812964/ https://www.ncbi.nlm.nih.gov/pubmed/24145165 http://dx.doi.org/10.1083/jcb.201304063 |
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