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Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements

Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital...

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Autores principales: Oddoux, Sarah, Zaal, Kristien J., Tate, Victoria, Kenea, Aster, Nandkeolyar, Shuktika A., Reid, Ericka, Liu, Wenhua, Ralston, Evelyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812964/
https://www.ncbi.nlm.nih.gov/pubmed/24145165
http://dx.doi.org/10.1083/jcb.201304063
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author Oddoux, Sarah
Zaal, Kristien J.
Tate, Victoria
Kenea, Aster
Nandkeolyar, Shuktika A.
Reid, Ericka
Liu, Wenhua
Ralston, Evelyn
author_facet Oddoux, Sarah
Zaal, Kristien J.
Tate, Victoria
Kenea, Aster
Nandkeolyar, Shuktika A.
Reid, Ericka
Liu, Wenhua
Ralston, Evelyn
author_sort Oddoux, Sarah
collection PubMed
description Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital, and superresolution microscopy, we find that muscle MTs are dynamic, growing at the typical speed of ∼9 µm/min, and forming small bundles that build a durable network. We also show that static Golgi elements, associated with the MT-organizing center proteins γ-tubulin and pericentrin, are major sites of muscle MT nucleation, in addition to the previously identified sites (i.e., nuclear membranes). These data give us a framework for understanding how muscle MTs organize and how they contribute to the pathology of muscle diseases such as Duchenne muscular dystrophy.
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spelling pubmed-38129642014-04-28 Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements Oddoux, Sarah Zaal, Kristien J. Tate, Victoria Kenea, Aster Nandkeolyar, Shuktika A. Reid, Ericka Liu, Wenhua Ralston, Evelyn J Cell Biol Research Articles Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital, and superresolution microscopy, we find that muscle MTs are dynamic, growing at the typical speed of ∼9 µm/min, and forming small bundles that build a durable network. We also show that static Golgi elements, associated with the MT-organizing center proteins γ-tubulin and pericentrin, are major sites of muscle MT nucleation, in addition to the previously identified sites (i.e., nuclear membranes). These data give us a framework for understanding how muscle MTs organize and how they contribute to the pathology of muscle diseases such as Duchenne muscular dystrophy. The Rockefeller University Press 2013-10-28 /pmc/articles/PMC3812964/ /pubmed/24145165 http://dx.doi.org/10.1083/jcb.201304063 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Oddoux, Sarah
Zaal, Kristien J.
Tate, Victoria
Kenea, Aster
Nandkeolyar, Shuktika A.
Reid, Ericka
Liu, Wenhua
Ralston, Evelyn
Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
title Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
title_full Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
title_fullStr Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
title_full_unstemmed Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
title_short Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements
title_sort microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at golgi elements
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812964/
https://www.ncbi.nlm.nih.gov/pubmed/24145165
http://dx.doi.org/10.1083/jcb.201304063
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