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The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1

Nuclear pore complexes (NPCs) are essential protein assemblies that span the nuclear envelope and establish nuclear–cytoplasmic compartmentalization. We have investigated mechanisms that control NPC number in mother and daughter cells during the asymmetric division of budding yeast. By simultaneousl...

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Autores principales: Colombi, Paolo, Webster, Brant M., Fröhlich, Florian, Lusk, C. Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812967/
https://www.ncbi.nlm.nih.gov/pubmed/24165936
http://dx.doi.org/10.1083/jcb.201305115
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author Colombi, Paolo
Webster, Brant M.
Fröhlich, Florian
Lusk, C. Patrick
author_facet Colombi, Paolo
Webster, Brant M.
Fröhlich, Florian
Lusk, C. Patrick
author_sort Colombi, Paolo
collection PubMed
description Nuclear pore complexes (NPCs) are essential protein assemblies that span the nuclear envelope and establish nuclear–cytoplasmic compartmentalization. We have investigated mechanisms that control NPC number in mother and daughter cells during the asymmetric division of budding yeast. By simultaneously tracking existing NPCs and newly synthesized NPC protomers (nups) through anaphase, we uncovered a pool of the central channel nup Nsp1 that is actively targeted to the bud in association with endoplasmic reticulum. Bud targeting required an intact actin cytoskeleton and the class V myosin, Myo2. Selective inhibition of cytoplasmic Nsp1 or inactivation of Myo2 reduced the inheritance of NPCs in daughter cells, leading to a daughter-specific loss of viability. Our data are consistent with a model in which Nsp1 releases a barrier that otherwise prevents NPC passage through the bud neck. It further supports the finding that NPC inheritance, not de novo NPC assembly, is primarily responsible for controlling NPC number in daughter cells.
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spelling pubmed-38129672014-04-28 The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1 Colombi, Paolo Webster, Brant M. Fröhlich, Florian Lusk, C. Patrick J Cell Biol Research Articles Nuclear pore complexes (NPCs) are essential protein assemblies that span the nuclear envelope and establish nuclear–cytoplasmic compartmentalization. We have investigated mechanisms that control NPC number in mother and daughter cells during the asymmetric division of budding yeast. By simultaneously tracking existing NPCs and newly synthesized NPC protomers (nups) through anaphase, we uncovered a pool of the central channel nup Nsp1 that is actively targeted to the bud in association with endoplasmic reticulum. Bud targeting required an intact actin cytoskeleton and the class V myosin, Myo2. Selective inhibition of cytoplasmic Nsp1 or inactivation of Myo2 reduced the inheritance of NPCs in daughter cells, leading to a daughter-specific loss of viability. Our data are consistent with a model in which Nsp1 releases a barrier that otherwise prevents NPC passage through the bud neck. It further supports the finding that NPC inheritance, not de novo NPC assembly, is primarily responsible for controlling NPC number in daughter cells. The Rockefeller University Press 2013-10-28 /pmc/articles/PMC3812967/ /pubmed/24165936 http://dx.doi.org/10.1083/jcb.201305115 Text en © 2013 Colombi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Colombi, Paolo
Webster, Brant M.
Fröhlich, Florian
Lusk, C. Patrick
The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1
title The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1
title_full The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1
title_fullStr The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1
title_full_unstemmed The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1
title_short The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1
title_sort transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of nsp1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812967/
https://www.ncbi.nlm.nih.gov/pubmed/24165936
http://dx.doi.org/10.1083/jcb.201305115
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