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Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression
Cyclin E regulates the cell cycle transition from G1 to S phase and is degraded before entry into G2 phase. Here we show that RhoBTB3, a Golgi-associated, Rho-related ATPase, regulates the S/G2 transition of the cell cycle by targeting Cyclin E for ubiquitylation. Depletion of RhoBTB3 arrested cells...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812982/ https://www.ncbi.nlm.nih.gov/pubmed/24145166 http://dx.doi.org/10.1083/jcb.201305158 |
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author | Lu, Albert Pfeffer, Suzanne R. |
author_facet | Lu, Albert Pfeffer, Suzanne R. |
author_sort | Lu, Albert |
collection | PubMed |
description | Cyclin E regulates the cell cycle transition from G1 to S phase and is degraded before entry into G2 phase. Here we show that RhoBTB3, a Golgi-associated, Rho-related ATPase, regulates the S/G2 transition of the cell cycle by targeting Cyclin E for ubiquitylation. Depletion of RhoBTB3 arrested cells in S phase, triggered Golgi fragmentation, and elevated Cyclin E levels. On the Golgi, RhoBTB3 bound Cyclin E as part of a Cullin3 (CUL3)-dependent RING–E3 ubiquitin ligase complex comprised of RhoBTB3, CUL3, and RBX1. Golgi association of this complex was required for its ability to catalyze Cyclin E ubiquitylation and allow normal cell cycle progression. These experiments reveal a novel role for a Ras superfamily member in catalyzing Cyclin E turnover during S phase, as well as an unexpected, essential role for the Golgi as a ubiquitylation platform for cell cycle control. |
format | Online Article Text |
id | pubmed-3812982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-38129822014-04-28 Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression Lu, Albert Pfeffer, Suzanne R. J Cell Biol Research Articles Cyclin E regulates the cell cycle transition from G1 to S phase and is degraded before entry into G2 phase. Here we show that RhoBTB3, a Golgi-associated, Rho-related ATPase, regulates the S/G2 transition of the cell cycle by targeting Cyclin E for ubiquitylation. Depletion of RhoBTB3 arrested cells in S phase, triggered Golgi fragmentation, and elevated Cyclin E levels. On the Golgi, RhoBTB3 bound Cyclin E as part of a Cullin3 (CUL3)-dependent RING–E3 ubiquitin ligase complex comprised of RhoBTB3, CUL3, and RBX1. Golgi association of this complex was required for its ability to catalyze Cyclin E ubiquitylation and allow normal cell cycle progression. These experiments reveal a novel role for a Ras superfamily member in catalyzing Cyclin E turnover during S phase, as well as an unexpected, essential role for the Golgi as a ubiquitylation platform for cell cycle control. The Rockefeller University Press 2013-10-28 /pmc/articles/PMC3812982/ /pubmed/24145166 http://dx.doi.org/10.1083/jcb.201305158 Text en © 2013 Lu and Pfeffer This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Lu, Albert Pfeffer, Suzanne R. Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression |
title | Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression |
title_full | Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression |
title_fullStr | Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression |
title_full_unstemmed | Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression |
title_short | Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression |
title_sort | golgi-associated rhobtb3 targets cyclin e for ubiquitylation and promotes cell cycle progression |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812982/ https://www.ncbi.nlm.nih.gov/pubmed/24145166 http://dx.doi.org/10.1083/jcb.201305158 |
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