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Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols
Gracilariopsis Persica (Rhodophyta) is one of the most abundant algae, introduced newly from the Indian Ocean. In this study, the main sterols of the algae have been isolated and identified. Separation and purification of the compounds was carried out on silica gel and sephadex LH(20) column chromat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813141/ https://www.ncbi.nlm.nih.gov/pubmed/24250511 |
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author | Saeidnia, Soodabeh Permeh, Parisa Gohari, Ahmad Reza Mashinchian-Moradi, Ali |
author_facet | Saeidnia, Soodabeh Permeh, Parisa Gohari, Ahmad Reza Mashinchian-Moradi, Ali |
author_sort | Saeidnia, Soodabeh |
collection | PubMed |
description | Gracilariopsis Persica (Rhodophyta) is one of the most abundant algae, introduced newly from the Indian Ocean. In this study, the main sterols of the algae have been isolated and identified. Separation and purification of the compounds was carried out on silica gel and sephadex LH(20) column chromatography (CC) and high performance liquid chromatography (HPLC) to obtain five pure compounds 1-5. Structural elucidation of the compounds was based on the data obtained from H-NMR, (13)C-NMR, DEPT and Mass spectroscopy. The separated compounds from Gp. Persica were identified as 22-dehydrocholesterol (1), cholesterol (2), stigmasterol (3), β-sitosterol (4) and fucosterol (5) based on the spectral data compared to those reported in literatures. Most of these sterols are noteworthy for their effectiveness in decreasing the plasma cholesterol, glucose and inflammation. The results of Brine Shrimp Cytotoxicity Assay indicated that the ethyl acetate extract of Gp. Persica showed a high cytotoxic effect against A. salina nauplii (LC(50) = 4 μg/mL). The methanol extract was no effective but the aqueous methanol extract was moderately effective (LC(50 )= 40 μg/mL) compared to berberine hydrochloride as a positive control (LC(50) = 26 μg/mL). |
format | Online Article Text |
id | pubmed-3813141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-38131412013-11-18 Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols Saeidnia, Soodabeh Permeh, Parisa Gohari, Ahmad Reza Mashinchian-Moradi, Ali Iran J Pharm Res Original Article Gracilariopsis Persica (Rhodophyta) is one of the most abundant algae, introduced newly from the Indian Ocean. In this study, the main sterols of the algae have been isolated and identified. Separation and purification of the compounds was carried out on silica gel and sephadex LH(20) column chromatography (CC) and high performance liquid chromatography (HPLC) to obtain five pure compounds 1-5. Structural elucidation of the compounds was based on the data obtained from H-NMR, (13)C-NMR, DEPT and Mass spectroscopy. The separated compounds from Gp. Persica were identified as 22-dehydrocholesterol (1), cholesterol (2), stigmasterol (3), β-sitosterol (4) and fucosterol (5) based on the spectral data compared to those reported in literatures. Most of these sterols are noteworthy for their effectiveness in decreasing the plasma cholesterol, glucose and inflammation. The results of Brine Shrimp Cytotoxicity Assay indicated that the ethyl acetate extract of Gp. Persica showed a high cytotoxic effect against A. salina nauplii (LC(50) = 4 μg/mL). The methanol extract was no effective but the aqueous methanol extract was moderately effective (LC(50 )= 40 μg/mL) compared to berberine hydrochloride as a positive control (LC(50) = 26 μg/mL). Shaheed Beheshti University of Medical Sciences 2012 /pmc/articles/PMC3813141/ /pubmed/24250511 Text en © 2012 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Saeidnia, Soodabeh Permeh, Parisa Gohari, Ahmad Reza Mashinchian-Moradi, Ali Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols |
title | Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols |
title_full | Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols |
title_fullStr | Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols |
title_full_unstemmed | Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols |
title_short | Gracilariopsis persica from Persian Gulf Contains Bioactive Sterols |
title_sort | gracilariopsis persica from persian gulf contains bioactive sterols |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813141/ https://www.ncbi.nlm.nih.gov/pubmed/24250511 |
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