Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi show...

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Autores principales: Mehdizadeh Kashani, Toktam, Vatanpour, Hossein, Zolfagharian, Hossein, Hooshdar Tehrani, Hasan, Heydari, Mohammad Hossein, Kobarfard, Farzad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813148/
https://www.ncbi.nlm.nih.gov/pubmed/24250552
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author Mehdizadeh Kashani, Toktam
Vatanpour, Hossein
Zolfagharian, Hossein
Hooshdar Tehrani, Hasan
Heydari, Mohammad Hossein
Kobarfard, Farzad
author_facet Mehdizadeh Kashani, Toktam
Vatanpour, Hossein
Zolfagharian, Hossein
Hooshdar Tehrani, Hasan
Heydari, Mohammad Hossein
Kobarfard, Farzad
author_sort Mehdizadeh Kashani, Toktam
collection PubMed
description Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 μM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity.
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spelling pubmed-38131482013-11-18 Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity Mehdizadeh Kashani, Toktam Vatanpour, Hossein Zolfagharian, Hossein Hooshdar Tehrani, Hasan Heydari, Mohammad Hossein Kobarfard, Farzad Iran J Pharm Res Original Article Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 μM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity. Shaheed Beheshti University of Medical Sciences 2012 /pmc/articles/PMC3813148/ /pubmed/24250552 Text en © 2012 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mehdizadeh Kashani, Toktam
Vatanpour, Hossein
Zolfagharian, Hossein
Hooshdar Tehrani, Hasan
Heydari, Mohammad Hossein
Kobarfard, Farzad
Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity
title Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity
title_full Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity
title_fullStr Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity
title_full_unstemmed Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity
title_short Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity
title_sort partial fractionation of venoms from two iranian vipers, echis carinatus and cerastes persicus fieldi and evaluation of their antiplatelet activity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813148/
https://www.ncbi.nlm.nih.gov/pubmed/24250552
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