Cargando…

In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum

Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinet...

Descripción completa

Detalles Bibliográficos
Autores principales: C. Jain, Satish, Pancholi, Boskey, Jain, Renuka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813163/
https://www.ncbi.nlm.nih.gov/pubmed/24250543
_version_ 1782289063119683584
author C. Jain, Satish
Pancholi, Boskey
Jain, Renuka
author_facet C. Jain, Satish
Pancholi, Boskey
Jain, Renuka
author_sort C. Jain, Satish
collection PubMed
description Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these calli were harvested, dried and extracted successively in pet, ether, methanol and water. Extracts were dried, weighed (%) and analyzed for their bioefficacies. Antimicrobial activities were determined using agar well diffusion and antioxidant potentials by DPPH and FRAP methods. Among all the test extracts, the extract of stem callus raised on IBA found to be more effective whereas it’s pet. ether extract showed appreciable activity against both the test bacteria and fungi (S. aureus- IZ 14.00 ± 0.57 mm and T. rubrum- 16.33 ± 0.32 mm), followed by methanol extract (S. aureus- IZ 13.00 ± 0.57 mm, A. niger and P. chrysogenum- IZ 16.66 mm in both). In antioxidant potentials, all aqueous extracts were more active where IBA and Kn extracts demonstrated 0.06 mg/mL IC50 value (% inhibition 93.30 and 92.70 respectively at 0.8 mg/mL concentration) with 366 ± 6.69 and 343 ± 3.34 ascorbic acid equivalent antioxidant potentials at 1 mg/mL concentration. Furthermore, the chemical profile of test extracts was carried out. The bioactive secondary metabolites, β-sitosterol and caffeic acid was isolated from culture tissue of 6 weeks-old callus, and their identification and confirmation was carried out by color reaction, TLC behavior and IR spectrum techniques.
format Online
Article
Text
id pubmed-3813163
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Shaheed Beheshti University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-38131632013-11-18 In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum C. Jain, Satish Pancholi, Boskey Jain, Renuka Iran J Pharm Res Original Article Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these calli were harvested, dried and extracted successively in pet, ether, methanol and water. Extracts were dried, weighed (%) and analyzed for their bioefficacies. Antimicrobial activities were determined using agar well diffusion and antioxidant potentials by DPPH and FRAP methods. Among all the test extracts, the extract of stem callus raised on IBA found to be more effective whereas it’s pet. ether extract showed appreciable activity against both the test bacteria and fungi (S. aureus- IZ 14.00 ± 0.57 mm and T. rubrum- 16.33 ± 0.32 mm), followed by methanol extract (S. aureus- IZ 13.00 ± 0.57 mm, A. niger and P. chrysogenum- IZ 16.66 mm in both). In antioxidant potentials, all aqueous extracts were more active where IBA and Kn extracts demonstrated 0.06 mg/mL IC50 value (% inhibition 93.30 and 92.70 respectively at 0.8 mg/mL concentration) with 366 ± 6.69 and 343 ± 3.34 ascorbic acid equivalent antioxidant potentials at 1 mg/mL concentration. Furthermore, the chemical profile of test extracts was carried out. The bioactive secondary metabolites, β-sitosterol and caffeic acid was isolated from culture tissue of 6 weeks-old callus, and their identification and confirmation was carried out by color reaction, TLC behavior and IR spectrum techniques. Shaheed Beheshti University of Medical Sciences 2012 /pmc/articles/PMC3813163/ /pubmed/24250543 Text en © 2012 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
C. Jain, Satish
Pancholi, Boskey
Jain, Renuka
In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
title In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
title_full In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
title_fullStr In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
title_full_unstemmed In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
title_short In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
title_sort in-vitro callus propagation and secondary metabolite quantification in sericostoma pauciflorum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813163/
https://www.ncbi.nlm.nih.gov/pubmed/24250543
work_keys_str_mv AT cjainsatish invitrocalluspropagationandsecondarymetabolitequantificationinsericostomapauciflorum
AT pancholiboskey invitrocalluspropagationandsecondarymetabolitequantificationinsericostomapauciflorum
AT jainrenuka invitrocalluspropagationandsecondarymetabolitequantificationinsericostomapauciflorum