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In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum
Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinet...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813163/ https://www.ncbi.nlm.nih.gov/pubmed/24250543 |
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author | C. Jain, Satish Pancholi, Boskey Jain, Renuka |
author_facet | C. Jain, Satish Pancholi, Boskey Jain, Renuka |
author_sort | C. Jain, Satish |
collection | PubMed |
description | Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these calli were harvested, dried and extracted successively in pet, ether, methanol and water. Extracts were dried, weighed (%) and analyzed for their bioefficacies. Antimicrobial activities were determined using agar well diffusion and antioxidant potentials by DPPH and FRAP methods. Among all the test extracts, the extract of stem callus raised on IBA found to be more effective whereas it’s pet. ether extract showed appreciable activity against both the test bacteria and fungi (S. aureus- IZ 14.00 ± 0.57 mm and T. rubrum- 16.33 ± 0.32 mm), followed by methanol extract (S. aureus- IZ 13.00 ± 0.57 mm, A. niger and P. chrysogenum- IZ 16.66 mm in both). In antioxidant potentials, all aqueous extracts were more active where IBA and Kn extracts demonstrated 0.06 mg/mL IC50 value (% inhibition 93.30 and 92.70 respectively at 0.8 mg/mL concentration) with 366 ± 6.69 and 343 ± 3.34 ascorbic acid equivalent antioxidant potentials at 1 mg/mL concentration. Furthermore, the chemical profile of test extracts was carried out. The bioactive secondary metabolites, β-sitosterol and caffeic acid was isolated from culture tissue of 6 weeks-old callus, and their identification and confirmation was carried out by color reaction, TLC behavior and IR spectrum techniques. |
format | Online Article Text |
id | pubmed-3813163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-38131632013-11-18 In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum C. Jain, Satish Pancholi, Boskey Jain, Renuka Iran J Pharm Res Original Article Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these calli were harvested, dried and extracted successively in pet, ether, methanol and water. Extracts were dried, weighed (%) and analyzed for their bioefficacies. Antimicrobial activities were determined using agar well diffusion and antioxidant potentials by DPPH and FRAP methods. Among all the test extracts, the extract of stem callus raised on IBA found to be more effective whereas it’s pet. ether extract showed appreciable activity against both the test bacteria and fungi (S. aureus- IZ 14.00 ± 0.57 mm and T. rubrum- 16.33 ± 0.32 mm), followed by methanol extract (S. aureus- IZ 13.00 ± 0.57 mm, A. niger and P. chrysogenum- IZ 16.66 mm in both). In antioxidant potentials, all aqueous extracts were more active where IBA and Kn extracts demonstrated 0.06 mg/mL IC50 value (% inhibition 93.30 and 92.70 respectively at 0.8 mg/mL concentration) with 366 ± 6.69 and 343 ± 3.34 ascorbic acid equivalent antioxidant potentials at 1 mg/mL concentration. Furthermore, the chemical profile of test extracts was carried out. The bioactive secondary metabolites, β-sitosterol and caffeic acid was isolated from culture tissue of 6 weeks-old callus, and their identification and confirmation was carried out by color reaction, TLC behavior and IR spectrum techniques. Shaheed Beheshti University of Medical Sciences 2012 /pmc/articles/PMC3813163/ /pubmed/24250543 Text en © 2012 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article C. Jain, Satish Pancholi, Boskey Jain, Renuka In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum |
title |
In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum |
title_full |
In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum |
title_fullStr |
In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum |
title_full_unstemmed |
In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum |
title_short |
In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum |
title_sort | in-vitro callus propagation and secondary metabolite quantification in sericostoma pauciflorum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813163/ https://www.ncbi.nlm.nih.gov/pubmed/24250543 |
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