Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations

Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 s...

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Detalles Bibliográficos
Autores principales: Brechtel, Teal M., Tyner, Molly, Tandon, Ritesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813188/
https://www.ncbi.nlm.nih.gov/pubmed/24158558
http://dx.doi.org/10.1128/genomeA.00901-13
Descripción
Sumario:Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 single-base substitutions were discovered in the passaged genome compared to the reference sequence (KF493877).

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