Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations

Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 s...

Descripción completa

Detalles Bibliográficos
Autores principales: Brechtel, Teal M., Tyner, Molly, Tandon, Ritesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813188/
https://www.ncbi.nlm.nih.gov/pubmed/24158558
http://dx.doi.org/10.1128/genomeA.00901-13
_version_ 1782289068690767872
author Brechtel, Teal M.
Tyner, Molly
Tandon, Ritesh
author_facet Brechtel, Teal M.
Tyner, Molly
Tandon, Ritesh
author_sort Brechtel, Teal M.
collection PubMed
description Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 single-base substitutions were discovered in the passaged genome compared to the reference sequence (KF493877).
format Online
Article
Text
id pubmed-3813188
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-38131882013-10-31 Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations Brechtel, Teal M. Tyner, Molly Tandon, Ritesh Genome Announc Viruses Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 single-base substitutions were discovered in the passaged genome compared to the reference sequence (KF493877). American Society for Microbiology 2013-10-24 /pmc/articles/PMC3813188/ /pubmed/24158558 http://dx.doi.org/10.1128/genomeA.00901-13 Text en Copyright © 2013 Brechtel et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Viruses
Brechtel, Teal M.
Tyner, Molly
Tandon, Ritesh
Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations
title Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations
title_full Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations
title_fullStr Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations
title_full_unstemmed Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations
title_short Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations
title_sort complete genome sequence of a ul96 mutant cytomegalovirus towne-bac (bacterial artificial chromosome) isolate passaged in fibroblasts to allow accumulation of compensatory mutations
topic Viruses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813188/
https://www.ncbi.nlm.nih.gov/pubmed/24158558
http://dx.doi.org/10.1128/genomeA.00901-13
work_keys_str_mv AT brechteltealm completegenomesequenceofaul96mutantcytomegalovirustownebacbacterialartificialchromosomeisolatepassagedinfibroblaststoallowaccumulationofcompensatorymutations
AT tynermolly completegenomesequenceofaul96mutantcytomegalovirustownebacbacterialartificialchromosomeisolatepassagedinfibroblaststoallowaccumulationofcompensatorymutations
AT tandonritesh completegenomesequenceofaul96mutantcytomegalovirustownebacbacterialartificialchromosomeisolatepassagedinfibroblaststoallowaccumulationofcompensatorymutations

Ejemplares similares