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Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS

Weichang’an (WCA) pill, a traditional Chinese patent medicine consisting of ten Chinese medicinal herbs, has been used to treat irritable bowel syndrome and functional dyspepsia for several decades. In this study, twelve bioactive constituents in the methanol extract of WCA were accurately identifie...

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Autores principales: Zhang, Jingze, Gao, Wenyuan, Liu, Zhen, Zhang, Zhidan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813220/
https://www.ncbi.nlm.nih.gov/pubmed/24250567
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author Zhang, Jingze
Gao, Wenyuan
Liu, Zhen
Zhang, Zhidan
author_facet Zhang, Jingze
Gao, Wenyuan
Liu, Zhen
Zhang, Zhidan
author_sort Zhang, Jingze
collection PubMed
description Weichang’an (WCA) pill, a traditional Chinese patent medicine consisting of ten Chinese medicinal herbs, has been used to treat irritable bowel syndrome and functional dyspepsia for several decades. In this study, twelve bioactive constituents in the methanol extract of WCA were accurately identified since MS/MS fragmentation behavior of the references and the standards by using HPLC-DAD-ESI-MS/MS analysis and a reliable and accurate method for the simultaneous determination was developed. Twelve active components including costunolide and dehydrodehydrocostus lactone from the principal herb Radix Aucklandiae; naringin, hesperidin and neohesperidin from Fructus Aurantii; magnolol and honokiol from the ministerial herbs Cortex Magnoliae officinalis; aloe-emodin, rhein, emodin, chrysophanol and physcion from adjunctive and messenger herb Radix et Rhizoma Rhei were analyzed in the samples. The chromatographic separation was performed on a Kromasil C(18 )column with gradient elution of acetonitrile-methanol and 1.0% acetic acid water. In this condition, linearity, inter- and intra-day precision and accuracy were within acceptable ranges. The developed method showed satisfactory precision and accuracy with overall intra- and inter-day variations of 0.68-1.33% and 0.67-2.05% respectively, and the overall recoveries of 97.54-102.69% for twelve compounds. The proposed approach was successfully applied as a powerful tool for the quality control of WCA pill.
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spelling pubmed-38132202013-11-18 Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS Zhang, Jingze Gao, Wenyuan Liu, Zhen Zhang, Zhidan Iran J Pharm Res Original Article Weichang’an (WCA) pill, a traditional Chinese patent medicine consisting of ten Chinese medicinal herbs, has been used to treat irritable bowel syndrome and functional dyspepsia for several decades. In this study, twelve bioactive constituents in the methanol extract of WCA were accurately identified since MS/MS fragmentation behavior of the references and the standards by using HPLC-DAD-ESI-MS/MS analysis and a reliable and accurate method for the simultaneous determination was developed. Twelve active components including costunolide and dehydrodehydrocostus lactone from the principal herb Radix Aucklandiae; naringin, hesperidin and neohesperidin from Fructus Aurantii; magnolol and honokiol from the ministerial herbs Cortex Magnoliae officinalis; aloe-emodin, rhein, emodin, chrysophanol and physcion from adjunctive and messenger herb Radix et Rhizoma Rhei were analyzed in the samples. The chromatographic separation was performed on a Kromasil C(18 )column with gradient elution of acetonitrile-methanol and 1.0% acetic acid water. In this condition, linearity, inter- and intra-day precision and accuracy were within acceptable ranges. The developed method showed satisfactory precision and accuracy with overall intra- and inter-day variations of 0.68-1.33% and 0.67-2.05% respectively, and the overall recoveries of 97.54-102.69% for twelve compounds. The proposed approach was successfully applied as a powerful tool for the quality control of WCA pill. Shaheed Beheshti University of Medical Sciences 2013 /pmc/articles/PMC3813220/ /pubmed/24250567 Text en © 2013 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhang, Jingze
Gao, Wenyuan
Liu, Zhen
Zhang, Zhidan
Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS
title Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS
title_full Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS
title_fullStr Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS
title_full_unstemmed Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS
title_short Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang’an Pill by HPLC-DAD-ESI-MS/MS
title_sort identification and simultaneous determination of twelve active components in the methanol extract of traditional medicine weichang’an pill by hplc-dad-esi-ms/ms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813220/
https://www.ncbi.nlm.nih.gov/pubmed/24250567
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