Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve

Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated lino...

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Detalles Bibliográficos
Autores principales: O'Connell, Kerry Joan, Motherway, Mary O'Connell, Hennessey, Alan A, Brodhun, Florian, Ross, R Paul, Feussner, Ivo, Stanton, Catherine, Fitzgerald, Gerald F, van Sinderen, Douwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813531/
https://www.ncbi.nlm.nih.gov/pubmed/23851389
http://dx.doi.org/10.4161/bioe.24159
Descripción
Sumario:Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

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